|Positive WB detected in||human brain tissue, HepG2 cells, mouse brain tissue, mouse liver tissue, rat liver tissue|
|Positive IHC detected in||human liver tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15838-1-AP targets GSTT1 in IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||hamster, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GSTT1 fusion protein Ag8588|
|Full Name||glutathione S-transferase theta 1|
|Calculated molecular weight||240aa,27 kDa|
|Observed molecular weight||28 kDa, 30 kDa|
|GenBank accession number||BC007065|
|Gene ID (NCBI)||2952|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.
J Occup Health
A trial to find appropriate animal models of dichloropropane-induced cholangiocarcinoma based on the hepatic distribution of glutathione S-transferases.
J Toxicol Sci
Modifying effects of 1,2-dichloropropane on N-nitrosobis(2-oxopropyl)amine-induced cholangiocarcinogenesis in male Syrian hamsters.
Int J Clin Exp Pathol
Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan.
Drug Metab Dispos
Evaluation of hepatic glutathione transferase Mu 1 and Theta 1 activities in humans and mice using genotype information.
Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-dichloropropane in Male Syrian Hamsters and B6C3F1 Mice.