GSTT1 Antibody

GSTT1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15838-1-AP

Synonyms

Glutathione transferase T1 1, GST class theta 1, GSTT1



Tested Applications

Positive WB detected inhuman brain tissue, HepG2 cells, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15838-1-AP targets GSTT1 in IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species hamster, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTT1 fusion protein Ag8588
Full Name glutathione S-transferase theta 1
Calculated molecular weight 240aa,27 kDa
Observed molecular weight 28 kDa, 30 kDa
GenBank accession number BC007065
Gene symbol GSTT1
Gene ID (NCBI) 2952
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.

Protocols

Product Specific Protocols
WB protocol for GSTT1 antibody 15838-1-APDownload protocol
IHC protocol for GSTT1 antibody 15838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Occup Health

A trial to find appropriate animal models of dichloropropane-induced cholangiocarcinoma based on the hepatic distribution of glutathione S-transferases.

Authors - Lingyi Zhang
mouseIHC

J Toxicol Sci

Modifying effects of 1,2-dichloropropane on N-nitrosobis(2-oxopropyl)amine-induced cholangiocarcinogenesis in male Syrian hamsters.

Authors - Min Gi
mouseIHC

Int J Clin Exp Pathol

Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan.

Authors - Yasunori Sato
humanWB

Drug Metab Dispos

Evaluation of hepatic glutathione transferase Mu 1 and Theta 1 activities in humans and mice using genotype information.

Authors - Arakawa Shingo S
hamsterIHC

Toxicol Sci

Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-dichloropropane in Male Syrian Hamsters and B6C3F1 Mice.

Authors - Min Wei