H2AFY2 Polyclonal antibody

H2AFY2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17030-1-AP

Synonyms

Core histone macro H2A.2, H2A histone family, member Y2, H2AFY2, histone, Histone macroH2A2, macroH2A2, mH2A2



Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, mouse kidney tissue
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

17030-1-AP targets H2AFY2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen H2AFY2 fusion protein Ag10487
Full Name H2A histone family, member Y2
Calculated Molecular Weight 372 aa, 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC016172
Gene Symbol H2AFY2
Gene ID (NCBI) 55506
RRIDAB_10638483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for H2AFY2 antibody 17030-1-APDownload protocol
IP protocol for H2AFY2 antibody 17030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Physiol

MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR-155.

Authors - Zhenzhen Li
mouseWB

Cell Physiol Biochem

Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6.

Authors - Zhenzhen Li
mouseWB

Immunol Cell Biol

ING4 alleviated lipopolysaccharide-induced inflammation by regulating the NF-κB pathway via a direct interaction with SIRT1.

Authors - Yunshu Yang
mouseWB

Arch Biochem Biophys

ROR alpha protects against LPS-induced inflammation by down-regulating SIRT1/NF-kappa B pathway.

Authors - Shichao Han
mouseWB

J Cell Physiol

MCPIP1 alleviated lipopolysaccharide-induced liver injury by regulating SIRT1 via modulation of microRNA-9.

Authors - Shichao Han