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HKDC1 Polyclonal antibody

HKDC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25874-1-AP

Synonyms

hexokinase domain containing 1, HKDC1, Putative hexokinase HKDC1

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using HKDC1 Polyclonal antibody (25874-1-AP)

    WB analysis of A549 using 25874-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25874-1-AP (HKDC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using HKDC1 Polyclonal antibody (25874-1-AP)

    IP experiment of mouse skeletal muscle using 25874-1-AP

    IP result of anti-HKDC1 (IP:25874-1-AP, 4ug; Detection:25874-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using HKDC1 Polyclonal antibody (25874-1-AP)

    IHC staining of human skeletal muscle using 25874-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25874-1-AP (HKDC1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using HKDC1 Polyclonal antibody (25874-1-AP)

    IHC staining of human skeletal muscle using 25874-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25874-1-AP (HKDC1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using HKDC1 Polyclonal antibody (25874-1-AP)

    IHC staining of mouse skeletal muscle using 25874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25874-1-AP (HKDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using HKDC1 Polyclonal antibody (25874-1-AP)

    IHC staining of mouse skeletal muscle using 25874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25874-1-AP (HKDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25874-1-AP is HKDC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HKDC1 fusion protein Ag23045
Full Name hexokinase domain containing 1
Calculated Molecular Weight 917 aa, 103 kDa
Observed Molecular Weight 102 kDa, 82-90 kDa
GenBank Accession NumberBC110504
Gene Symbol HKDC1
Gene ID (NCBI) 80201
RRIDAB_2880279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HKDC1 antibody 25874-1-APDownload protocol
IHC protocol for HKDC1 antibody 25874-1-APDownload protocol
IP protocol for HKDC1 antibody 25874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Virol Sin

Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.

Authors - Yingying Song
View Article
humanIHC,IF

Int J Mol Sci

Mitochondria-Related Transcriptome Characterization Associated with the Immune Microenvironment, Therapeutic Response and Survival Prediction in Pancreatic Cancer

Authors - Jia Dong
View Article
mouseWB,IHC

J Mol Histol

New insights into the treatment of polycystic ovary syndrome: HKDC1 promotes the growth of ovarian granulocyte cells by regulating mitochondrial function and glycolysis

Authors - Peiwei Cong
  • KD Validated
View Article