HKDC1 Polyclonal antibody

HKDC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25874-1-AP

Synonyms

hexokinase domain containing 1, HKDC1, Putative hexokinase HKDC1



Tested Applications

Positive WB detected inA549 cells,
Positive IP detected inmouse skeletal muscle tissue,
Positive IHC detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25874-1-AP is HKDC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HKDC1 fusion protein Ag23045
Full Name hexokinase domain containing 1
Calculated molecular weight 917 aa, 103 kDa
Observed molecular weight 102 kDa, 82-90 kDa
GenBank accession numberBC110504
Gene symbol HKDC1
Gene ID (NCBI) 80201
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HKDC1 antibody 25874-1-APDownload protocol
IHC protocol for HKDC1 antibody 25874-1-APDownload protocol
IP protocol for HKDC1 antibody 25874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols