HLA class I (HLA-B) Polyclonal antibody

HLA class I (HLA-B) Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17260-1-AP

Synonyms

AS, HLA B, HLA B 7301, HLA B73, HLAB, HLA-B, HLAC, MHC class I antigen B*7, SPDA1



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, MCF-7 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman spleen tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human stomach cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17260-1-AP targets HLA class I (HLA-B) in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA class I (HLA-B) fusion protein Ag10947
Full Name major histocompatibility complex, class I, B
Calculated molecular weight 362 aa, 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC013187
Gene symbol HLA-B
Gene ID (NCBI) 3106
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
WB protocol for HLA class I (HLA-B) antibody 17260-1-APDownload protocol
IHC protocol for HLA class I (HLA-B) antibody 17260-1-APDownload protocol
IF protocol for HLA class I (HLA-B) antibody 17260-1-APDownload protocol
IP protocol for HLA class I (HLA-B) antibody 17260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (09-16-2020)

This antibody recognises a specific band at the expected MW.

  • Applications: Western Blot