Filter: AllWBIPIF/ICC

WB analysis using 14797-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 14797-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14797-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 14797-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 14797-1-AP
IP result of anti-HMBS (IP:14797-1-AP, 4ug; Detection:14797-1-AP 1:2000) with HEK-293 cells lysate 1200 ug.
IF Staining of MCF-7 using 14797-1-AP
Immunofluorescent analysis of MCF-7 cells, using HMBS antibody 14797-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HeLa using 14797-1-AP
Immunofluorescent analysis of Hela cells, using HMBS antibody 14797-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HepG2 using 14797-1-AP
Immunofluorescent analysis of HepG2 cells, using HMBS antibody 14797-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

HMBS Polyclonal antibody

HMBS Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 14797-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

EC:2.5.1.61, hydroxymethylbilane synthase, PBG D, PBGD, PBG-D

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, A375 cells, Raji cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HEK-293 cells
Positive IF/ICC detected in	MCF-7 cells, Hela cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14797-1-AP targets HMBS in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6509 Product name: Recombinant human HMBS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-361 aa of BC000520 Sequence: MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH Predict reactive species
Full Name	hydroxymethylbilane synthase
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	39-42 kDa
GenBank Accession Number	BC000520
Gene Symbol	HMBS
Gene ID (NCBI)	3145
RRID	AB_2117447
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08397
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for HMBS antibody 14797-1-AP	Download protocol
IP protocol for HMBS antibody 14797-1-AP	Download protocol
WB protocol for HMBS antibody 14797-1-AP	Download protocol