HMBS Antibody

HMBS Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14797-1-AP

Synonyms

HMBS, hydroxymethylbilane synthase, PBG D, PBGD, Porphobilinogen deaminase, Pre uroporphyrinogen synthase, UPS



Tested Applications

Positive WB detected inHeLa cells, A375 cells, HEK-293 cells, Raji cells
Positive IP detected inHEK-293 cells
Positive IF detected inMCF-7 cells, Hela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14797-1-AP targets HMBS in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMBS fusion protein Ag6509
Full Name hydroxymethylbilane synthase
Calculated molecular weight 39 kDa
Observed molecular weight 39-42 kDa
GenBank accession number BC000520
Gene symbol HMBS
Gene ID (NCBI) 3145
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HMBS antibody 14797-1-APDownload protocol
IF protocol for HMBS antibody 14797-1-APDownload protocol
IP protocol for HMBS antibody 14797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols