HMBS Antibody

HMBS Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12H3

Cat no : 67456-1-Ig

Synonyms

HMBS, hydroxymethylbilane synthase, PBG D, PBGD, Porphobilinogen deaminase, Pre uroporphyrinogen synthase, UPS



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells, HepG2 cells, Caco-2 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67456-1-Ig targets HMBS in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HMBS fusion protein Ag6594
Full Name hydroxymethylbilane synthase
Calculated molecular weight 39 kDa
Observed molecular weight 39-42 kDa
GenBank accession number BC000520
Gene symbol HMBS
Gene ID (NCBI) 3145
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HMBS antibody 67456-1-IgDownload protocol
IHC protocol for HMBS antibody 67456-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols