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  • KD/KO Validated

HNF1B Polyclonal antibody

HNF1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12533-1-AP

Synonyms

TCF-2, TCF2, HNF-1-beta, HNF-1B, HNF 1 beta



Tested Applications

Positive WB detected inL02 cells, Jurkat cells, MDCK cells
Positive IHC detected inhuman renal cell carcinoma tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12533-1-AP targets HNF1B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNF1B fusion protein Ag3232
Full Name HNF1 homeobox B
Calculated Molecular Weight 557 aa, 61 kDa
Observed Molecular Weight 58-60 kDa
GenBank Accession NumberBC017714
Gene Symbol HNF1B
Gene ID (NCBI) 6928
RRIDAB_2116758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. This is a rabbit polyclonal antibody raised against part chain of N-terminus of human HNF1B. HNF1B exists some isoforms with MV 61 kDa, 58 kDa, 46 kDa and 44 kDa.

Protocols

Product Specific Protocols
WB protocol for HNF1B antibody 12533-1-APDownload protocol
IHC protocol for HNF1B antibody 12533-1-APDownload protocol
IF protocol for HNF1B antibody 12533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Elife

Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.

Authors - Marko Z Nikolić
humanWB,IHC

Oncogene

HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer.

Authors - Jianqing Wang
  • KD Validated
mouseIF

Development

Prox1 ablation in hepatic progenitors causes defective hepatocyte specification and increases biliary cell commitment.

Authors - Asha Seth
pigIF

Sci Rep

Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.

Authors - Phillip L Lewis
humanWB,IHC

J Cell Mol Med

HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer.

Authors - Wei Lu
mouseIHC

Sci Rep

ATM-deficiency increases genomic instability and metastatic potential in a mouse model of pancreatic cancer.

Authors - Yiannis Drosos