CTBP2 Polyclonal antibody

CTBP2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10346-1-AP

Synonyms

C terminal binding protein 2, CTBP2

---

Validation Data Gallery

• 

  WB analysis using 10346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10346-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 10346-1-AP

  IP Result of anti-CTBP2 (IP:10346-1-AP, 4ug; Detection:10346-1-AP 1:500) with mouse heart tissue lysate 3200ug.

• 

  IF Staining of HeLa using 10346-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CTBP2 antibody (10346-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, rat brain tissue
• Positive IP detected in: mouse heart tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 4 publications below

Product Information

10346-1-AP targets CTBP2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, gerbil, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTBP2 fusion protein Ag0352
• Full Name: C-terminal binding protein 2
• Calculated molecular weight: 49 kDa
• Observed molecular weight: 49 kDa
• GenBank accession number: BC002486
• Gene symbol: CTBP2
• Gene ID (NCBI): 1488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-Terminal binding protein 2 (CTBP2) is a transcriptional repressor. It contains a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. This protein is thought to bind to the C-terminus of the adenovirus E1A proteins. Studies in mice suggested that this protein is involved in transcriptional repression. CTBP2 is expressed in all tissues tested, with a higher level of expression in the heart, skeletal muscle, and pancreas. The gene of CTBP2 is mapped to human chromosome 21q21.3.

Publications

Species	Application	Title
zebrafish	IF	PLoS Biol; Hair cell identity establishes labeled lines of directional mechanosensation.; Authors - Marta Lozano-Ortega
gerbil	IF	J Neurosci; Tonotopic variation in the calcium dependence of neurotransmitter release and vesicle pool replenishment at mammalian auditory ribbon synapses.; Authors - Johnson Stuart L SL
mouse	IF	Neural Plast; Specific Influences of Early Acoustic Environments on Cochlear Hair Cells in Postnatal Mice.; Authors - Aoshuang Chang
zebrafish	IF	Genet Mol Res; MicroRNA-200 family members are weakly expressed in the neurosensory epithelia of the developing zebrafish (Danio rerio) inner ear.; Authors - J T Du