CTBP2 Polyclonal antibody

CTBP2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 10346-1-AP

Synonyms

C terminal binding protein 2, CTBP2



Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, rat brain tissue
Positive IP detected inmouse heart tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 4 publications below

Product Information

10346-1-AP targets CTBP2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, gerbil, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTBP2 fusion protein Ag0352
Full Name C-terminal binding protein 2
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC002486
Gene symbol CTBP2
Gene ID (NCBI) 1488
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-Terminal binding protein 2 (CTBP2) is a transcriptional repressor. It contains a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. This protein is thought to bind to the C-terminus of the adenovirus E1A proteins. Studies in mice suggested that this protein is involved in transcriptional repression. CTBP2 is expressed in all tissues tested, with a higher level of expression in the heart, skeletal muscle, and pancreas. The gene of CTBP2 is mapped to human chromosome 21q21.3.

Protocols

Product Specific Protocols
WB protocol for CTBP2 antibody 10346-1-APDownload protocol
IF protocol for CTBP2 antibody 10346-1-APDownload protocol
IP protocol for CTBP2 antibody 10346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishIF

PLoS Biol

Hair cell identity establishes labeled lines of directional mechanosensation.

Authors - Marta Lozano-Ortega
gerbilIF

J Neurosci

Tonotopic variation in the calcium dependence of neurotransmitter release and vesicle pool replenishment at mammalian auditory ribbon synapses.

Authors - Johnson Stuart L SL
mouseIF

Neural Plast

Specific Influences of Early Acoustic Environments on Cochlear Hair Cells in Postnatal Mice.

Authors - Aoshuang Chang
zebrafishIF

Genet Mol Res

MicroRNA-200 family members are weakly expressed in the neurosensory epithelia of the developing zebrafish (Danio rerio) inner ear.

Authors - J T Du