CTBP2 Polyclonal antibody

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  WB analysis using 10346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10346-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10346-1-AP

  IP result of anti-CTBP2 (IP:10346-1-AP, 4ug; Detection:10346-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human pancreas cancer using 10346-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10346-1-AP (CTBP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10346-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10346-1-AP (CTBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10346-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10346-1-AP (CTBP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10346-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CTBP2 antibody (10346-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10346-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CTBP2 (10346-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

CTBP2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10346-1-AP

Publications(11)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

C-terminal-binding protein 2

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, rat brain tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human pancreas cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Product Information

10346-1-AP targets CTBP2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish, gerbil
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTBP2 fusion protein Ag0352
• Full Name: C-terminal binding protein 2
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC002486
• Gene Symbol: CTBP2
• Gene ID (NCBI): 1488
• RRID: AB_2261202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56545
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-Terminal binding protein 2 (CTBP2) is a transcriptional repressor. It contains a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. This protein is thought to bind to the C-terminus of the adenovirus E1A proteins. Studies in mice suggested that this protein is involved in transcriptional repression. CTBP2 is expressed in all tissues tested, with a higher level of expression in the heart, skeletal muscle, and pancreas. The gene of CTBP2 is mapped to human chromosome 21q21.3.

Publications

Species	Application	Title
zebrafish	IF	PLoS Biol; Hair cell identity establishes labeled lines of directional mechanosensation.; Authors - Marta Lozano-Ortega
gerbil	IF	J Neurosci; Tonotopic variation in the calcium dependence of neurotransmitter release and vesicle pool replenishment at mammalian auditory ribbon synapses.; Authors - Johnson Stuart L SL
mouse	IF	Neural Plast; Specific Influences of Early Acoustic Environments on Cochlear Hair Cells in Postnatal Mice.; Authors - Aoshuang Chang
mouse	WB	Proteome Sci; Proteomic analysis of neonatal mouse hearts shows PKA functions as a cardiomyocyte replication regulator; Authors - Lizhi Hu
zebrafish	IF	Genet Mol Res; MicroRNA-200 family members are weakly expressed in the neurosensory epithelia of the developing zebrafish (Danio rerio) inner ear.; Authors - J T Du
mouse	WB	Front Physiol; Ling-gui-zhu-gan granules reduces obesity and ameliorates metabolic disorders by inducing white adipose tissue browning in obese mice; Authors - Yuxiu Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (02-04-2025)

high specificity and sensitivity, providing reliable and reproducible results

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: ROs