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  • KD/KO Validated

CTBP2 Polyclonal antibody

CTBP2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10346-1-AP

Synonyms

C-terminal-binding protein 2



Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, rat brain tissue
Positive IP detected inmouse heart tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10346-1-AP targets CTBP2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish, gerbil
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTBP2 fusion protein Ag0352
Full Name C-terminal binding protein 2
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002486
Gene Symbol CTBP2
Gene ID (NCBI) 1488
RRIDAB_2261202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-Terminal binding protein 2 (CTBP2) is a transcriptional repressor. It contains a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. This protein is thought to bind to the C-terminus of the adenovirus E1A proteins. Studies in mice suggested that this protein is involved in transcriptional repression. CTBP2 is expressed in all tissues tested, with a higher level of expression in the heart, skeletal muscle, and pancreas. The gene of CTBP2 is mapped to human chromosome 21q21.3.

Protocols

Product Specific Protocols
WB protocol for CTBP2 antibody 10346-1-APDownload protocol
IF protocol for CTBP2 antibody 10346-1-APDownload protocol
IP protocol for CTBP2 antibody 10346-1-APDownload protocol
FC protocol for CTBP2 antibody 10346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishIF

PLoS Biol

Hair cell identity establishes labeled lines of directional mechanosensation.

Authors - Marta Lozano-Ortega
gerbilIF

J Neurosci

Tonotopic variation in the calcium dependence of neurotransmitter release and vesicle pool replenishment at mammalian auditory ribbon synapses.

Authors - Johnson Stuart L SL
mouseIF

Neural Plast

Specific Influences of Early Acoustic Environments on Cochlear Hair Cells in Postnatal Mice.

Authors - Aoshuang Chang
mouseWB

Proteome Sci

Proteomic analysis of neonatal mouse hearts shows PKA functions as a cardiomyocyte replication regulator

Authors - Lizhi Hu
zebrafishIF

Genet Mol Res

MicroRNA-200 family members are weakly expressed in the neurosensory epithelia of the developing zebrafish (Danio rerio) inner ear.

Authors - J T Du
humanWB,IHC,IF

J Clin Invest

The CTBP2-PCIF1 complex regulates m6Am modification of mRNA in head and neck squamous cell carcinoma

Authors - Kang Li
  • KO Validated