Tested Applications
| Positive WB detected in | mouse kidney tissue, Y79 cells, mouse pancreas tissue, rat kidney tissue, mouse heart tissue |
| Positive IHC detected in | human kidney tissue, human pancreas cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
12035-1-AP targets KIF12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2707 Product name: Recombinant human KIF12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-334 aa of BC010626 Sequence: MQRTFAWLLDRVQHLGAPVTLRASYLEIYNEQVRDLLSLGSPRPLPVRWNKTRGFYVEQLRVVEFGSLEALMELLQTGLSRRRNSAHTLNQASSRSHALLTLYISRQTAQQMPSVDPGEPPVGGKLCFVDLAGSEKVAATGSRGELMLEANSINRSLLALGHCISLLLDPQRKQSHIPFRDSKLTKLLADSLGGRGVTLMVACVSPSAQCLPETLSTLRYASRAQRVTTRPQAPKSPVAKQPQRLETEMLQLQEENRRLQFQLDQMDCKASGLSGARVAWAQRNLYGMLQEFMLENERLRKEKSQLQNSRDLAQNEQRILAQQVHALERRLLSA Predict reactive species |
| Full Name | kinesin family member 12 |
| Calculated Molecular Weight | 646 aa, 71 kDa |
| Observed Molecular Weight | 55-70 kDa |
| GenBank Accession Number | BC010626 |
| Gene Symbol | KIF12 |
| Gene ID (NCBI) | 113220 |
| RRID | AB_2280813 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96FN5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIF12(kinesin family member 12) is a member of the kinesin superfamily (KIF), which is a microtubule-dependent ATP-driven molecular motor, and its core is involved in the regulation of organelle transport, cell division and metabolic enzyme degradation. Its functional defects are closely related to bile duct diseases, liver diseases and tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIF12 antibody 12035-1-AP | Download protocol |
| WB protocol for KIF12 antibody 12035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Kidney Int Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney. | ||
Breast Cancer Res Treat Specific kinesin expression profiles associated with taxane resistance in basal-like breast cancer. | ||
J Proteomics Proteomic analysis of podocyte exosome-enriched fraction from normal human urine. | ||
J Pediatr KIF12 Variants and Disturbed Hepatocyte Polarity in Children With a Phenotypic Spectrum of Cholestatic Liver Disease. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Huxley (Verified Customer) (07-28-2025) | Did not work for western blotting on cell lysates. Bands on western blot were the same in KIF12 knock-out cells (the knockout of KIF12 had been confirmed by genotyping) and in cells overexpressing KIF12-GFP as in control cells, so they must be nonspecific bands. However, ProteinTech's customer service was excellent and they promptly issued a refund for this.
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