Polycystin 2 Polyclonal antibody

Polycystin 2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19126-1-AP

Synonyms

APKD2, PC2, PKD2, PKD4, Polycystin 2, Polycystwin, R48321

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Validation Data Gallery

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  WB analysis using 19126-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 19126-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19126-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19126-1-AP

  IP result of anti-Polycystin 2 (IP:19126-1-AP, 3ug; Detection:19126-1-AP 1:500) with mouse testis tissue lysate 8000ug.

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  IHC staining of human kidney using 19126-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19126-1-AP (Polycystin 2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 19126-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19126-1-AP (Polycystin 2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of MDCK using 19126-1-AP

  Immunofluorescent analysis of MDCK cells using 19126-1-AP (Polycystin 2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, human kidney tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

19126-1-AP targets Polycystin 2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: polycystic kidney disease 2 (autosomal dominant)
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 109 kDa
• GenBank Accession Number: NM_000297
• Gene Symbol: PKD2
• Gene ID (NCBI): 5311
• RRID: AB_10641050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polycystin 2 (PKD2), the product of the gene mutated in type 2 autosomal dominant polycystic kidney disease, belongs to the polycystin family. PKD2 is a ~110-kDa six-transmembrane channel protein with cytoplasmic N- and C-termini. This protein functions as a Ca2+-activated intracellular Ca2+ release channel in the endoplasmic reticulum. It is also present in the plasma membrane, where it functions as a nonselective cation channel. In addition, PKD2 expression has been documented in the primary cilium of kidney epithelial cells, where it is believed to have an essential role in mediating Ca2+ entry in response to flow rate changes, suggesting that it may be part of a mechanosensing machinery residing in the primary cilium. (PMID: 16135816; 10497221)

Publications

Species	Application	Title
mouse	WB	Front Mol Biosci; The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries.; Authors - Xin Hou
human	IHC	Hum Pathol; Down-regulation of polycystin in lymphatic malformations: Possible role in the proliferation of lymphatic endothelial cells.; Authors - Jian-Gang Ren
rat	WB	Cell Physiol Biochem; iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.; Authors - Xiaomei Liu
mouse	IF,IHC,WB	Graefes Arch Clin Exp Ophthalmol; TRPP2 is located in the primary cilia of human non-pigmented ciliary epithelial cells; Authors - Wenxu Zheng