Tested Applications
| Positive WB detected in | DU 145 cells, HepG2 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human testis tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17637-1-AP targets EHBP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11916 Product name: Recombinant human EHBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 813-1160 aa of BC067215 Sequence: FCNRQLSDQQDEERRRQLRERARQLIAEARSGVKMSELPSYGEMAAEKLKERSKASGEQNSKLVDLKLKKLLEVQPQVANSPSSAAQKAVTESSEQDMKSGTEDLRTERLQKTTERFRNPVVFSKDSTVRKTQLQSFSQYIENRPEMKRQRSIQEDTKKGNEEKAAITETQRKPSEDEVLNKGFKDTSQYVVGELAALENEQKQIDTRAALVEKRLRYLMDTGRNTEEEEAMMQEWFMLVNKKNALIRRMNQLSLLEKEHDLERRYELLNRELRAMLAIEDWQKTEAQKRREQLLLDELVALVNKRDALVRDLDAQEKQAEEEDEHLERTLEQNKGKMAKKEEKCVLQ Predict reactive species |
| Full Name | EH domain binding protein 1 |
| Calculated Molecular Weight | 132 kDa |
| Observed Molecular Weight | 160-180 kDa |
| GenBank Accession Number | BC067215 |
| Gene Symbol | EHBP1 |
| Gene ID (NCBI) | 23301 |
| RRID | AB_2097216 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NDI1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EHBP1 antibody 17637-1-AP | Download protocol |
| IHC protocol for EHBP1 antibody 17637-1-AP | Download protocol |
| IP protocol for EHBP1 antibody 17637-1-AP | Download protocol |
| WB protocol for EHBP1 antibody 17637-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EHBP1 Polyclonal antibody (Cat# 17637-1-AP) has 11 citations. These appear in journals including Cell Metabolism, Nature Communications, Molecular Cell, Science Advances, PNAS, Journal of Cell Biology, EMBO Reports, Frontiers in Cell and Developmental Biology, Journal of Neuroscience, Journal of Cell Science, and STAR Protocols. Research fields span liver fibrosis/steatohepatosis, membrane/caveolae organization, oncogenic Ras/mTORC2 signaling, autophagy and lipid droplets, lysosomal homeostasis, Wnt sorting, viral infection, dendritic arbor formation, ciliary function, and hepatocyte LDLR detection.
| Species | Application | Title |
|---|---|---|
Cell Metab EHBP1 suppresses liver fibrosis in metabolic dysfunction-associated steatohepatitis | ||
Nat Commun The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane | ||
Sci Adv A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets.
| ||
Proc Natl Acad Sci U S A LRRK2 and its substrate Rab GTPases are sequentially targeted onto stressed lysosomes and maintain their homeostasis.
| ||
J Cell Biol VPS13C regulates phospho-Rab10-mediated lysosomal function in human dopaminergic neurons |
