Cadherin-16 Polyclonal antibody

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  WB analysis using 15107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15107-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15107-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15107-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Cadherin-16 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15107-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

cadherin 16, KSP cadherin, Cadherin-16, CDH16, Kidney specific cadherin, Ksp cadherin

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human kidney tissue, HEK-293 cells, rat kidney tissue
• Positive IHC detected in: human kidney tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 6 publications below

Product Information

15107-1-AP targets Cadherin-16 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4343

  Product name: Recombinant human CDH16 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 20-350 aa of BC027912

  Sequence: AQPAELSVEVPENYGGNFPLYLTKLPLPREGAEGQIVLSGDSGKATEGPFAMDPDSGFLLVTRALDREEQAEYQLQVTLEMQDGHVLWGPQPVLVHVKDENDQVPHFSQAIYRARLSRGTRPGIPFLFLEASDRDEPGTANSDLRFHILSQAPAQPSPDMFQLEPRLGALALSPKGSTSLDHALERTYQLLVQVKDMGDQASGHQATATVEVSIIESTWVSLEPIHLAENLKVLYPHHMAQVHWSGGDVHYHLESHPPGPFEVNAEGNLYVTRELDREAQAEYLLQVRAQNSHGEDYAAPLELHVLVMDENDNVPICPPRDPTVSIPELSP

• Full Name: cadherin 16, KSP-cadherin
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC027912
• Gene Symbol: Cadherin-16
• Gene ID (NCBI): 1014
• RRID: AB_10596931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75309
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherin-16 (CDH16), also known as kidney-specific cadherin (Ksp-cadherin), is a tissue-specific member of the cadherin family of adhesion molecules (PMID: 7615566). It is a glycoprotein expressed by tubular epithelial cells in the kidney. Cadherin-16 expression is down-regulated in renal cell carcinoma, suggesting a role in tumor suppression (PMID: 15886705).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Int Immunopharmacol; Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion; Authors - Zhi Zhao
mouse	WB,IF	Front Cell Dev Biol; Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition.; Authors - Yanhong Zhang
mouse	WB,IF	J Cell Sci; Pax8 controls thyroid follicular polarity through Cadherin-16.; Authors - Petrina Koumarianou ; KD Validated
human	WB	J Cancer; TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers.; Authors - Jiaqi Dai
human	WB	Oncol Lett; Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.; Authors - Xiaolin Yang
mouse	IF	Cell Death Dis; Blocking connexin 43 and its promotion of ATP release from renal tubular epithelial cells ameliorates renal fibrosis.; Authors - Huzi Xu