Cadherin-16 Polyclonal antibody

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Cadherin-16 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15107-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

cadherin 16, KSP cadherin, Cadherin-16, CDH16, Kidney specific cadherin, Ksp cadherin

• 

  WB analysis using 15107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 15107-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human kidney using 15107-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 15107-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15107-1-AP (Cadherin-16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 15107-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15107-1-AP (Cadherin-16 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human kidney tissue, HEK-293 cells, rat kidney tissue
• Positive IHC detected in: human kidney tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15107-1-AP targets Cadherin-16 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4343

  Product name: Recombinant human CDH16 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 20-350 aa of BC027912

  Sequence: AQPAELSVEVPENYGGNFPLYLTKLPLPREGAEGQIVLSGDSGKATEGPFAMDPDSGFLLVTRALDREEQAEYQLQVTLEMQDGHVLWGPQPVLVHVKDENDQVPHFSQAIYRARLSRGTRPGIPFLFLEASDRDEPGTANSDLRFHILSQAPAQPSPDMFQLEPRLGALALSPKGSTSLDHALERTYQLLVQVKDMGDQASGHQATATVEVSIIESTWVSLEPIHLAENLKVLYPHHMAQVHWSGGDVHYHLESHPPGPFEVNAEGNLYVTRELDREAQAEYLLQVRAQNSHGEDYAAPLELHVLVMDENDNVPICPPRDPTVSIPELSP

• Full Name: cadherin 16, KSP-cadherin
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC027912
• Gene Symbol: Cadherin-16
• Gene ID (NCBI): 1014
• RRID: AB_10596931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75309
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherin-16 (CDH16), also known as kidney-specific cadherin (Ksp-cadherin), is a tissue-specific member of the cadherin family of adhesion molecules (PMID: 7615566). It is a glycoprotein expressed by tubular epithelial cells in the kidney. Cadherin-16 expression is down-regulated in renal cell carcinoma, suggesting a role in tumor suppression (PMID: 15886705).

Publications

What published studies show

The anti-Cadherin-16 antibody (Cat# 15107-1-AP) has 11 total citations. These appear in journals including Nature Communications, Cell Death & Disease, Journal of Medicinal Chemistry, International Immunopharmacology, Journal of Clinical Endocrinology & Metabolism, Frontiers in Cell and Developmental Biology, Journal of Cell Science, Journal of Cancer, Oncology Letters, and bioRxiv. Research fields span renal fibrosis and injury, diabetic kidney disease, acute kidney injury, ferroptosis, thyroid nodules and carcinoma, epithelial-mesenchymal transition, and cancer cell biology.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Commun; Targeting the transmembrane cytokine co-receptor neuropilin-1 in distal tubules improves renal injury and fibrosis; Authors - Yinzheng Li
mouse	IF	Cell Death Dis; Blocking connexin 43 and its promotion of ATP release from renal tubular epithelial cells ameliorates renal fibrosis.; Authors - Huzi Xu
mouse,human	WB	Cell Death Dis; Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease.; Authors - Shan Song
mouse	IF	J Med Chem; NIR-II Light-Controlled Photosynthetic Activation via an Upconversion Nanoplatform for Targeted Bioenergetic Therapy in Acute Kidney Injury.; Authors - Xiaojuan Hu
mouse	IF	Int Immunopharmacol; Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion; Authors - Zhi Zhao
human	IF	J Clin Endocrinol Metab; Somatic DICER1-Mutant Benign Thyroid Nodules in Adults: A Group of Follicular Nodular Disease With Continuous Growth; Authors - Lingyang Meng