HNRNPA2B1 Antibody 14813-1-AP

Tested Applications

Applications:

ELISA, WB, IP, IHC, IF
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HNRNPA2B1 Antibody WB HL-60 cells 14813-1-AP

HL-60 cells were subjected to SDS PAGE followed by western blot with 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

HNRNPA2B1 Antibody IP mouse liver tissue 14813-1-AP

IP Result of anti-HNRNPA2B1 (IP:14813-1-AP, 4ug; Detection:14813-1-AP 1:500) with mouse liver tissue lysate 4000ug.

HNRNPA2B1 Antibody IHC human cervical cancer tissue 14813-1-AP

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 14813-1-AP( HNRNPA2B1 Antibody) at dilution of 1:50 (under 40x lens)

HNRNPA2B1 Antibody IHC human lymphoma tissue 14813-1-AP

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 14813-1-AP( HNRNPA2B1 Antibody) at dilution of 1:50 (under 40x lens)

HNRNPA2B1 Antibody IF HeLa cells 14813-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:

human, mouse, rat

Positive WB detected in:

HL-60 cells, A549 cells, HeLa cells, HL-60 cells, Jurkat cells, K-562 cells, Raji cells

Positive IP detected in:

mouse liver tissue

Positive IHC detected in:

human lymphoma tissue, human cervical cancer tissue

Positive IF detected in:

HeLa cells

Recommended dilution:

WB : 1:500-1:3000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:20-1:200

IF : 1:50-1:500

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

HNRNPA2B1 fusion protein ag6563

Full name:

heterogeneous nuclear ribonucleoprotein A2/B1

Calculated molecular weight:

37 kDa

Observed molecular weight:

36 kDa and 38 kDa

GenBank accession number:

BC000506

Gene ID (NCBI):

3181

Gene symbol

HNRNPA2B1

Synonyms

DKFZp779B0244, FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPA2B1, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1

Background

HnRNPA2B1 in one of heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA and participate in growth regulation and carcinogenesis. It has a role in packaging nascent mRNA, alternative splicing, tycoplamic RNA trafficking, translation and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encode two protein, HNRNPA2 (38kd) and HNRNPB1 (36kd), by alternative splicing.

Showing 1- [5] of [5] Publications

Author:		Guo Rong R
Pubmed ID:		23658645
Journal:		PLoS One
Title:		HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells.
Application:		WB,IHC
KD validated
Species:		human

Author:		Kyoung-In Cho
Pubmed ID:		24403063
Journal:		J Biol Chem
Title:		Differential Loss of Prolyl-Isomerase or Chaperone Activity of Ran-binding protein 2 (Ranbp2) Unveils Distinct Physiological Roles of Its Cyclophilin Domain in Proteostasis.
Application:		WB,IF

Species:		mouse

Author:		Hiroaki Suzuki
Pubmed ID:		25378556
Journal:		Hum Mol Genet
Title:		Nuclear TDP-43 causes neuronal toxicity by escaping from the inhibitory regulation by hnRNPs.
Application:		WB

Species:		human

Author:		Kyoungin Cho
Pubmed ID:		26030368
Journal:		ACS Chem Neurosci
Title:		Targeting the Cyclophilin Domain of Ran-binding Protein 2 (Ranbp2) with Novel Small Molecules to Control the Proteostasis of STAT3, hnRNPA2B1 and M-Opsin.
Application:		WB

Species:		human

Author:		Chunhe Yang
Pubmed ID:		27226232
Journal:		Scand J Immunol
Title:		Bioinformatics driven new immune target discovery in disease.
Application:

Species:

WB Figures

HNRNPA2B1 Antibody WB A549 cells 14813-1-AP

A549 cells were subjected to SDS PAGE followed by western blot with 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

HNRNPA2B1 Antibody WB HeLa cells 14813-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

HL-60 cells were subjected to SDS PAGE followed by western blot with 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

HL-60 cells were subjected to SDS PAGE followed by western blot with 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HNRNPA2B1 Antibody WB Jurkat cells 14813-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

HNRNPA2B1 Antibody WB K-562 cells 14813-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HNRNPA2B1 Antibody WB Raji cells 14813-1-AP

Raji cells were subjected to SDS PAGE followed by western blot with 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 14813-1-AP( HNRNPA2B1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 14813-1-AP( HNRNPA2B1 Antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human lymphoma using 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human lymphoma using 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 14813-1-AP( HNRNPA2B1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 14813-1-AP( HNRNPA2B1 Antibody) at dilution of 1:50 (under 40x lens)

IP Figures

IP Result of anti-HNRNPA2B1 (IP:14813-1-AP, 4ug; Detection:14813-1-AP 1:500) with mouse liver tissue lysate 4000ug.

IF Figures

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 14813-1-AP(HNRNPA2B1 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

HNRNPA2B1 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 14813-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [5] of [5] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

HNRNPA2B1 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 14813-1-AP

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Tested Applications

Product Information

Immunogen Information

Background