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Tested Applications
| Positive WB detected in | A549 cells, A431 cells, HL-60 cells, HSC-T6 cells, HeLa cells, K-562 cells |
| Positive IHC detected in | human colon tissue, human lymphoma tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 11 publications below |
| WB | See 53 publications below |
| IHC | See 12 publications below |
| IF | See 10 publications below |
| IP | See 2 publications below |
| CoIP | See 3 publications below |
Product Information
14813-1-AP targets HNRNPA2B1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HNRNPA2B1 fusion protein Ag6563 Predict reactive species |
| Full Name | heterogeneous nuclear ribonucleoprotein A2/B1 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 36 kDa and 38 kDa |
| GenBank Accession Number | BC000506 |
| Gene Symbol | HNRNPA2B1 |
| Gene ID (NCBI) | 3181 |
| RRID | AB_2279638 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22626 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HnRNPA2B1 is one of the heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation, and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encodes two proteins, HNRNPA2 (36kd) and HNRNPB1 (38kd), by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HNRNPA2B1 antibody 14813-1-AP | Download protocol |
| IHC protocol for HNRNPA2B1 antibody 14813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer The therapeutically actionable long non-coding RNA 'T-RECS' is essential to cancer cells' survival in NRAS/MAPK-driven melanoma | ||
Cell Mol Immunol Inhibition of METTL3 in macrophages provides protection against intestinal inflammation | ||
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Mol Cancer CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner | ||
Theranostics LINC00982-encoded protein PRDM16-DT regulates CHEK2 splicing to suppress colorectal cancer metastasis and chemoresistance
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