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HOXD8 Polyclonal antibody

HOXD8 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 18588-1-AP

Synonyms

homeobox D8, Homeobox protein Hox 4E, Homeobox protein Hox 5.4, Homeobox protein Hox D8, HOX4, HOX4E, HOX5.4, HOXD8

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using HOXD8 Polyclonal antibody (18588-1-AP)

    WB analysis of A375 using 18588-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 18588-1-AP (HOXD8 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HeLa cells using HOXD8 Polyclonal antibody (18588-1-AP)

    WB analysis of HeLa using 18588-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18588-1-AP (HOXD8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HOXD8 Polyclonal antibody (18588-1-AP)

    WB analysis of HEK-293 using 18588-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18588-1-AP (HOXD8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using HOXD8 Polyclonal antibody (18588-1-AP)

    WB analysis of A375 using 18588-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 18588-1-AP (HOXD8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA375 cells, HEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18588-1-AP is HOXD8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOXD8 fusion protein Ag13245
Full Name homeobox D8
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC038709
Gene Symbol HOXD8
Gene ID (NCBI) 3234
RRIDAB_2878554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HOXD8 antibody 18588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols