Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, human placenta tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10978-1-AP targets HSD17B2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1411 Product name: Recombinant human HSD17B2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-387 aa of BC009581 Sequence: MSTFFSDTAWICLAVPTVLCGTVFCKYKKSSGQLWSWMVCLAGLCAVCLLILSPFWGLILFSVSCFLMYTYLSGQELLPVDQKAVLVTGGDCGLGHALCKYLDELGFTVFAGVLNENGPGAEELRRTCSPRLSVLQMDITKPVQIKDAYSKVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVTKTFLPLLRKSKGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLELSKWGIKVASIQPGGFLTNIAGTSDKWEKLEKDILDHLPAEVQEDYGQDYILAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICLAHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT Predict reactive species |
| Full Name | hydroxysteroid (17-beta) dehydrogenase 2 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC009581 |
| Gene Symbol | HSD17B2 |
| Gene ID (NCBI) | 3294 |
| RRID | AB_2279863 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSD17B2(Estradiol 17-beta-dehydrogenase 2) is also named as 17-beta-HSD 2,20-alpha-HSD,EDH17B2 and belongs to the short-chain dehydrogenases/reductases (SDR) family.Based on the pivotal role of HSD17B2 gene product in the estrogen metabolism pathway, it may be considered as a putative candidate gene that could possibly explain a fraction of the remaining familial breast cancer risk and it is unlikely to play a role as a high penetrance gene in breast cancer predisposition(PMID:18372405).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HSD17B2 antibody 10978-1-AP | Download protocol |
| WB protocol for HSD17B2 antibody 10978-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
10978-1-AP is an anti-HSD17B2 antibody with 40 total citations. Citations appear in journals including Cancer Research, Fertility and Sterility, Breast Cancer Research, International Journal of Molecular Sciences, Journal of Translational Medicine, British Journal of Cancer, Endocrinology, and Biochemical and Biophysical Research Communications. Research fields span steroid hormone metabolism, breast cancer, prostate cancer, endometriosis, reproductive toxicology, environmental toxicology, liver disease, and colorectal cancer.
| Species | Application | Title |
|---|---|---|
Cancer Res Hypoxia-Reoxygenation Couples 3βHSD1 Enzyme and Cofactor Upregulation to Facilitate Androgen Biosynthesis and Hormone Therapy Resistance in Prostate Cancer | ||
Phytomedicine Paridis rhizoma total saponins ameliorate abnormal uterine bleeding via regulating Proteus mirabilis 2024-mediated estrogen metabolism in the gut-reproductive axis. | ||
J Hazard Mater Exposure to 1-nitropyrene after weaning induces anxiety-like behavior partially by inhibiting steroid hormone synthesis in prefrontal cortex | ||
Part Fibre Toxicol Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway. | ||
J Transl Med Intratumoral localization and activity of 17beta-hydroxysteroid dehydrogenase type 1 in non-small cell lung cancer: a potent prognostic factor. | ||
Fertil Steril Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome. |
