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  • KD/KO Validated

Hsc70 Polyclonal antibody

Hsc70 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10654-1-AP

Synonyms

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, HSPA8



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, C6 cells, COLO 320 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10654-1-AP targets Hsc70 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hsc70 fusion protein Ag1018
Full Name heat shock 70kDa protein 8
Calculated molecular weight 70 kDa
Observed molecular weight 70-73 kDa
GenBank accession numberBC007276
Gene symbol HSPA8
Gene ID (NCBI) 3312
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson’s disease, indicating its implication in neurodegenerative disease. (21832061)

Protocols

Product Specific Protocols
WB protocol for Hsc70 antibody 10654-1-APDownload protocol
IHC protocol for Hsc70 antibody 10654-1-APDownload protocol
IF protocol for Hsc70 antibody 10654-1-APDownload protocol
IP protocol for Hsc70 antibody 10654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Chaperone-mediated autophagy is involved in the execution of ferroptosis.

Authors - Zheming Wu
humanWB

Cell Stress Chaperones

PKCι counteracts oxidative stress by regulating Hsc70 in an esophageal cancer cell line.

Authors - Wang Bo-Shi BS
humanWB

Biochem Biophys Res Commun

Exosomes derived from breast cancer lung metastasis subpopulations promote tumor self-seeding.

Authors - Hehai Huang
mouseWB

J Agric Food Chem

A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.

Authors - Cheng Liu
humanWB

PLoS One

hERG Potassium Channel Blockage by Scorpion Toxin BmKKx2 Enhances Erythroid Differentiation of Human Leukemia Cells K562.

Authors - Jian Ma
humanWB

Mar Drugs

An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.

Authors - Su Ching-Chyuan CC

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

I had a very strong and specific Hsc70 band at ~70 kDa. Definitely one of the best antibodies I have used for Hsp70 family proteins.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart
Hsc70 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:10654-1-AP)
FH

Mayur (Verified Customer) (04-30-2019)

Great antibody. Detects the HSC70 expression in the H4 cells very clearly.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H4
Hsc70 Antibody Western Blot, validation (1:1000 dilution) in H4 (Cat no:10654-1-AP)