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HTR6 Polyclonal antibody

HTR6 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23561-1-AP

Synonyms

5 HT 6, 5 HT6, 5 HT6R, 5 hydroxytryptamine receptor 6, HTR6, Serotonin receptor 6

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human brain tissue using HTR6 Polyclonal antibody (23561-1-AP)

    IHC staining of human brain using 23561-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23561-1-AP (HTR6 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using HTR6 Polyclonal antibody (23561-1-AP)

    IHC staining of human brain using 23561-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23561-1-AP (HTR6 Antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using HTR6 Polyclonal antibody (23561-1-AP)

    IF Staining of mouse brain using 23561-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using HTR6 antibody (23561-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using HTR6 Polyclonal antibody (23561-1-AP)

    IF Staining of mouse brain using 23561-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using HTR6 antibody (23561-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using HTR6 Polyclonal antibody (23561-1-AP)

    IF Staining of rat brain using 23561-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using HTR6 antibody (23561-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using HTR6 Polyclonal antibody (23561-1-AP)

    IF Staining of rat brain using 23561-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using HTR6 antibody (23561-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 23561-1-AP is HTR6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HTR6 fusion protein Ag15396
Full Name 5-hydroxytryptamine (serotonin) receptor 6
Calculated Molecular Weight 440 aa, 47 kDa
GenBank Accession NumberBC074996
Gene Symbol HTR6
Gene ID (NCBI) 3362
RRIDAB_2879296
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for HTR6 antibody 23561-1-APDownload protocol
IF protocol for HTR6 antibody 23561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cureus

Possible Role of 5-Hydroxytryptamine (5-HT) Receptor on Human Sperm Motility Regulation

Authors - Maya Omote
View Article