IARS Antibody

IARS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26942-1-AP

Synonyms

IARS, IARS1, IleRS, ILRS, IRS, Isoleucine tRNA ligase, isoleucyl tRNA synthetase, PRO0785



Tested Applications

Positive WB detected inK-562 cells, U2OS cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman testis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 26942-1-AP immunogen is Fusion Protein IARS protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IARS fusion protein Ag25686
Full Name isoleucyl-tRNA synthetase
Calculated molecular weight 144 kDa
Observed molecular weight 145 kDa
GenBank accession number BC065552
Gene symbol IARS
Gene ID (NCBI) 3376
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IARS antibody 26942-1-APDownload protocol
IHC protocol for IARS antibody 26942-1-APDownload protocol
IP protocol for IARS antibody 26942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols