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IFIT1 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 23247-1-AP

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Con: 37 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

IFIT1 Antibody WB HeLa cells 23247-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 23247-1-AP( IFIT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

IFIT1 Antibody WB LPS treated RAW 264.7 cells 23247-1-AP

LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 23247-1-AP( IFIT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

IFIT1 Antibody WB U-937 cells 23247-1-AP

U-937 cells were subjected to SDS PAGE followed by western blot with 23247-1-AP( IFIT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

IFIT1 Antibody IHC human breast cancer tissue 23247-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer slide using 23247-1-AP( IFIT1 Antibody) at dilution of 1:50

IFIT1 Antibody IHC human breast cancer tissue 23247-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer slide using 23247-1-AP( IFIT1 Antibody) at dilution of 1:50

IFIT1 Antibody IHC human liver cancer tissue 23247-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer slide using 23247-1-AP( IFIT1 Antibody) at dilution of 1:50

IFIT1 Antibody IHC human liver cancer tissue 23247-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer slide using 23247-1-AP( IFIT1 Antibody) at dilution of 1:50

Species specificity:
human, mouse, rat
Positive WB detected in:
U-937 cells, HeLa cells, LPS treated RAW 264.7 cells
Positive IHC detected in:
human breast cancer tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen Affinity purified
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IFIT1 fusion protein Ag19691
Full name:
interferon-induced protein with tetratricopeptide repeats 1
Calculated molecular weight:
478aa,55 kDa
Observed molecular weight:
55 Kda
GenBank accession number:
BC007091
Gene ID (NCBI):
3434
Gene symbol
IFIT1
Synonyms
G10P1, GARG 16, IFI 56, IFI 56K, IFI56, IFIT 1, IFIT1, IFNAI1, ISG56, RNM561

Background

IFIT1 is, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.


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