IFIT1 Polyclonal antibody
IFIT1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 23247-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U-937 cells, HeLa cells, LPS treated RAW 264.7 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 6 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 23247-1-AP is IFIT1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IFIT1 fusion protein Ag19691 |
| Full Name | interferon-induced protein with tetratricopeptide repeats 1 |
| Calculated molecular weight | 478 aa, 55 kDa |
| Observed molecular weight | 55 Kda |
| GenBank accession number | BC007091 |
| Gene symbol | IFIT1 |
| Gene ID (NCBI) | 3434 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFIT1 is, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFIT1 antibody 23247-1-AP | Download protocol |
| IHC protocol for IFIT1 antibody 23247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci Mechanism of berberine in treating Helicobacter pylori induced chronic atrophic gastritis through IRF8-IFN-γ signaling axis suppressing. | ||
J Proteome Res Label-free Proteomic Analysis of Exosomes Secreted from THP-1-derived Macrophages Treated with IFN-α Identifies Antiviral Proteins Enriched in Exosomes. | ||
J Biol Chem A RIG-I-like receptor directs antiviral responses to a bunyavirus and is antagonized by virus-induced blockade of TRIM25-mediated ubiquitination. | ||
J Virol Herpes Simplex Virus 1 Tegument Protein UL41 Counteracts IFIT3 Antiviral Innate Immunity. |