|Positive WB detected in||HepG2 cells, K-562 cells|
|Positive IP detected in||ROS1728 cells|
|Positive IHC detected in||human lymphoma tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||K-562 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11727-3-AP targets IFITM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat samples.
|Tested Reactivity||human, rat|
|Cited Reactivity||goat, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFITM1 fusion protein Ag2320|
|Full Name||interferon induced transmembrane protein 1 (9-27)|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||14-17 kDa|
|GenBank accession number||BC000897|
|Gene ID (NCBI)||8519|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFITM1(interferon induced transmembrane protein), also named DSPA2a or interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection.
|Product Specific Protocols|
|WB protocol for IFITM1 antibody 11727-3-AP||Download protocol|
|IHC protocol for IFITM1 antibody 11727-3-AP||Download protocol|
|IP protocol for IFITM1 antibody 11727-3-AP||Download protocol|
|FC protocol for IFITM1 antibody 11727-3-AP||Download protocol|
|Click here to view our Standard Protocols|
IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection.
Cell Cycle Checkpoints Cooperate to Suppress DNA- and RNA-Associated Molecular Pattern Recognition and Anti-Tumor Immune Responses.
J Infect Dis
Interferon-Induced Transmembrane Protein-Mediated Inhibition of Host Cell Entry of Ebolaviruses.
Res Vet Sci
Infection of goats with goatpox virus triggers host antiviral defense through activation of innate immune signaling.
Biochem Biophys Res Commun
Antiviral Role of IFITM Proteins in African Swine Fever Virus Infection.