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  • KD/KO Validated

IFITM2-specific Monoclonal antibody

IFITM2-specific Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3D5F7

Cat no : 66137-1-Ig

Synonyms

DSPA2c, IFITM2



Tested Applications

Positive WB detected inHepG2 cells, recombinant protein
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66137-1-Ig is IFITM2-specific Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fusion Protein
Full Name interferon induced transmembrane protein 2 (1-8D)
Observed molecular weight 15 kDa
GenBank accession numberNM_006435
Gene symbol IFITM2
Gene ID (NCBI) 10581
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.

Protocols

Product Specific Protocols
WB protocol for IFITM2-specific antibody 66137-1-IgDownload protocol
FC protocol for IFITM2-specific antibody 66137-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Chem Biol

IFITM3 directly engages and shuttles incoming virus particles to lysosomes.

Authors - Jennifer S Spence
humanWB

Viruses

PMID: 32370187

Authors - Theodore A Gobillot
humanWB

Retrovirology

A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery.

Authors - Ferdinand Roesch
  • KO Validated
humanWB

Cell Microbiol

IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection.

Authors - Xun Zhu
humanWB

Sci Rep

IFITM proteins inhibit HIV-1 protein synthesis.

Authors - Wing-Yiu Jason Lee
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Authors - Guoli Shi