Tested Applications
| Positive WB detected in | HepG2 cells, recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66137-1-Ig targets IFITM2-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | interferon induced transmembrane protein 2 (1-8D) |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | NM_006435 |
| Gene Symbol | IFITM2 |
| Gene ID (NCBI) | 10581 |
| RRID | AB_2881536 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q01629 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM2-Specific antibody 66137-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cell Host Microbe IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion | ||
Nat Chem Biol IFITM3 directly engages and shuttles incoming virus particles to lysosomes. | ||
Sci Adv Restriction of HIV-1 infectivity by interferon and IFITM3 is counteracted by Nef. | ||
Mol Ther A modified cyclosporine enhances lentivector transduction ex vivo and in vivo by degrading IFITM3. | ||
Proc Natl Acad Sci U S A mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3. |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Claudia (Verified Customer) (06-20-2022) | The antibody worked very well for the analysis of IFITM2 expression in permanent cell lines.
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