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IFITM2-specific Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66137-1-Ig |CloneNo.: 3D5F7

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Tested Applications
Applications:
ELISA, FC, WB
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66137-1-Ig;recombinant protein protein

IFITM1 (Lane 1), IFITM2 (Lane 2) and IFITM3 (Lane 3) fusion proteins (20ng/lane) were subjected to SDS-PAGE followed by western blot with IFITM2 monoclonal antibody (66137-1-Ig) at dilution of 1:10,000.

66137-1-Ig;HepG2 cells

HepG2 cells were subjected to SDS PAGE followed by western blot with 66137-1-Ig(IFITM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

66137-1-Ig;HeLa cells

1X10^6 HeLa cells were stained with 0.2ug IFITM2-specific antibody (66137-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Species specificity:
human
Positive WB detected in:
HepG2 cells, recombinant protein
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Fusion Protein
Full name:
interferon induced transmembrane protein 2 (1-8D)
Calculated molecular weight:
Observed molecular weight:
15 kDa
GenBank accession number:
NM_006435
Gene ID (NCBI):
10581
Gene symbol
IFITM2
Synonyms
DSPA2c, IFITM2
Background

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.


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