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- KD/KO Validated
IFITM2-specific Monoclonal antibody
IFITM2-specific Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3D5F7
Cat no : 66137-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, recombinant protein |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
Product Information
The immunogen of 66137-1-Ig is IFITM2-specific Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Fusion Protein |
| Full Name | interferon induced transmembrane protein 2 (1-8D) |
| Observed molecular weight | 15 kDa |
| GenBank accession number | NM_006435 |
| Gene symbol | IFITM2 |
| Gene ID (NCBI) | 10581 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM2-specific antibody 66137-1-Ig | Download protocol |
| FC protocol for IFITM2-specific antibody 66137-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Chem Biol IFITM3 directly engages and shuttles incoming virus particles to lysosomes. | ||
Retrovirology A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery.
| ||
Cell Microbiol IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection. | ||
Proc Natl Acad Sci U S A mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3. |
