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  • KD/KO Validated

IGFBP2 Polyclonal antibody

IGFBP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11065-3-AP

Synonyms

BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2



Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11065-3-AP targets IGFBP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity chicken, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP2 fusion protein Ag1535
Full Name IGF binding protein 2
Calculated molecular weight 35 kDa
Observed molecular weight 33-36 kDa
GenBank accession numberBC009902
Gene symbol IGFBP2
Gene ID (NCBI) 3485
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INS-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degraded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers.

Protocols

Product Specific Protocols
WB protocol for IGFBP2 antibody 11065-3-APDownload protocol
IHC protocol for IGFBP2 antibody 11065-3-APDownload protocol
IP protocol for IGFBP2 antibody 11065-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenWB

Cells

MiR-34b-5p Mediates the Proliferation and Differentiation of Myoblasts by Targeting IGFBP2.

Authors - Zhijun Wang
  • KD Validated
chickenWB

Oxid Med Cell Longev

IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart.

Authors - Yafan Gong
chickenWB

Cell Signal

PMID: 28734787

Authors - Jie Yang
mouseWB

Nat Commun

Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.

Authors - Hyunho Kim