IGFBP2 Polyclonal antibody

IGFBP2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15699-1-AP

Synonyms



Tested Applications

Positive WB detected inMCF-7 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15699-1-AP targets IGFBP2 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP2 fusion protein Ag8327
Full Name IGF binding protein 2
Calculated molecular weight 36 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC004312
Gene symbol IGFBP2
Gene ID (NCBI) 3485
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degarded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.

Protocols

Product Specific Protocols
WB protocol for IGFBP2 antibody 15699-1-APDownload protocol
IHC protocol for IGFBP2 antibody 15699-1-APDownload protocol
IF protocol for IGFBP2 antibody 15699-1-APDownload protocol
Standard Protocols
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