IKBKB Antibody

IKBKB Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20979-1-AP

Synonyms

I kappa B kinase 2, I kappa B kinase beta, IKBKB, IKK B, IKK beta, IKK2, IKKB, IκB, NFKBIKB



Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inDU 145 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

20979-1-AP targets IKBKB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Calculated molecular weight 87 kDa
Observed molecular weight 80, 86, 87 kDa
GenBank accession number NM_001556
Gene symbol IKBKB
Gene ID (NCBI) 3551
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IKBKB, also named as NFKBIKB, IKK2 and IKKB, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. It also phosphorylates NCOA3. The antibody is specific to IKBKB. This antibody can identify 3 isoform of IKBKB with the molecular weight of 80, 86, 87 kDa.

Protocols

Product Specific Protocols
WB protocol for IKBKB antibody 20979-1-APDownload protocol
IHC protocol for IKBKB antibody 20979-1-APDownload protocol
IF protocol for IKBKB antibody 20979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neurochem Res

Intrathecal Delivery of ssAAV9-DAO Extends Survival in SOD1(G93A) ALS Mice.

Authors - Wan Wang
ratWB

Inflammation

PMID: 28744711

Authors - Hongfei Zhao
humanWB

Oncotarget

PMID: 26993770

Authors - Xueyuan Tang
ratWB

Redox Biol

Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro.

Authors - Dejuan Li