Filter: AllWBIHCIF-PIF/ICC

WB analysis using 18474-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18474-1-AP (IKBKG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 18474-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18474-1-AP (IKBKG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse lung using 18474-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18474-1-AP (IKBKG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 18474-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18474-1-AP (IKBKG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18474-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18474-1-AP (IKBKG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 18474-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18474-1-AP (IKBKG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 18474-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18474-1-AP (IKBKG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 18474-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 18474-1-AP (IKBKG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat lung using 18474-1-AP
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 18474-1-AP (IKBKG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse embryo using 18474-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 18474-1-AP (IKBKG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of K-562 using 18474-1-AP
Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IKBKG antibody (18474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

IKBKG Polyclonal antibody

Name: IKBKG Polyclonal antibody
Brand: Proteintech
SKU: 18474-1-AP
Rating: 5.00 (1 reviews)

IKBKG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18474-1-AP

Publications(33)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

AMCBX1, FIP3, FIP-3, Fip3p, I-kappa-B kinase subunit gamma

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (33)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, mouse brain tissue
Positive IHC detected in	human placenta tissue, mouse brain tissue, human lung tissue, mouse liver tissue, mouse lung tissue, rat liver tissue, rat lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	K-562 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 32 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 3 publications below
CoIP	See 2 publications below

Product Information

18474-1-AP targets IKBKG in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13358 Product name: Recombinant human IKBKG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 120-419 aa of BC012114 Sequence: QALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE Predict reactive species
Full Name	inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	48 kDa
GenBank Accession Number	BC012114
Gene Symbol	IKBKG
Gene ID (NCBI)	8517
RRID	AB_10646443
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y6K9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.

Protocols

Product Specific Protocols
IF protocol for IKBKG antibody 18474-1-AP	Download protocol
IHC protocol for IKBKG antibody 18474-1-AP	Download protocol
WB protocol for IKBKG antibody 18474-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Cell Biol Caspase inhibition prolongs inflammation by promoting a signaling complex with activated RIPK1. Authors - Xinyue Huang View Article
human	WB	Cell Death Differ IKK-β mediates hydrogen peroxide induced cell death through p85 S6K1. Authors - Jia C-H CH KD Validated View Article
human	WB	Mol Cell Proteomics Quantification of Dynamic Protein Interactions and Phosphorylation in LPS Signaling Pathway by SWATH-MS Authors - Xiurong Wu View Article
human	WB	Front Immunol PCBP1 interacts with the HTLV-1 Tax oncoprotein to potentiate NF-κB activation Authors - Rui Su View Article
human	WB	Mol Oncol RIP1 modulates death receptor mediated apoptosis and autophagy in macrophages. Authors - Zhenyu Yao View Article
human	WB,IF	FASEB J 3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche. Authors - Stefanie Inglis View Article