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  • KD/KO Validated

IKBKG Polyclonal antibody

IKBKG Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18474-1-AP

Synonyms

AMCBX1, FIP 3, FIP3, Fip3p, I kappa B kinase subunit gamma, IkB kinase subunit gamma, IKBKG, IKK gamma, IKKAP1, IKKG, IP, IP1, IP2, IPD2, NEMO, NF kappa B essential modifier, NF kappa B essential modulator



Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, mouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse embryo tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18474-1-AP is IKBKG Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IKBKG fusion protein Ag13358
Full Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC012114
Gene symbol IKBKG
Gene ID (NCBI) 8517
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.

Protocols

Product Specific Protocols
WB protocol for IKBKG antibody 18474-1-APDownload protocol
IHC protocol for IKBKG antibody 18474-1-APDownload protocol
IF protocol for IKBKG antibody 18474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Toxins (Basel)

Evaluation of the Liver Toxicity of Pterocephalus hookeri Extract via Triggering Necrosis.

Authors - Rui Wang
mouseWB

Mol Cell Proteomics

Quantification of dynamic protein interactions and phosphorylation in LPS signaling pathway by SWATH-MS.

Authors - Xiurong Wu
humanWB

J Mol Cell Biol

TRIM14 promotes endothelial activation via activating NF-κB signaling pathway.

Authors - Xuan Huang
  • KD Validated
humanWB,IP

Cells

KRAB-Zinc Finger Protein ZNF268a Deficiency Attenuates the Virus-Induced Pro-Inflammatory Response by Preventing IKK Complex Assembly.

Authors - Yi Liu
humanWB

Microbes Infect

SNW1 interacts with IKKγ to positively regulate antiviral innate immune responses against influenza A virus infection.

Authors - Qiao Zhang
mouseWB

Neurochem Res

Intrathecal Delivery of ssAAV9-DAO Extends Survival in SOD1(G93A) ALS Mice.

Authors - Wan Wang