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Tested Applications
| Positive WB detected in | C2C12 cells, EL-4 cells, THP-1 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85179-5-RR targets IL-4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | interleukin 4 |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC027514 |
| Gene Symbol | IL-4 |
| Gene ID (NCBI) | 16189 |
| RRID | AB_3744078 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07750 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IL-4 antibody 85179-5-RR | Download protocol |
| WB protocol for IL-4 antibody 85179-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Toxicology Chronic exposure to polystyrene microplastics induces renal fibrosis via ferroptosis | ||
Eur J Pharmacol Essential roles of IL-4 signaling in the prophylactic effect of gardiquimod on chronic stress-induced depression-like behaviors and neuroinflammatory responses. | ||
Biochem Biophys Res Commun PLCγ1 knockdown in BMSCs enhances bone repair via IL-4-JAK2-STAT3-mediated reparative macrophage polarization. | ||
Int Immunopharmacol TREM2 regulates neuroinflammation by SYK-dependent inhibition of BTK activation to improve perioperative neurocognitive dysfunction. | ||
J Nutr Biochem High-fat diet exacerbates atopic dermatitis through alterations in the gut microbiome. |
