IL-10 Monoclonal antibody

IL-10 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

8E4B6

Cat no : 60269-1-Ig

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF



Tested Applications

Positive WB detected inag14870
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60269-1-Ig is IL-10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IL-10 fusion protein Ag14870
Full Name interleukin 10
Calculated molecular weight 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC104252
Gene symbol IL10
Gene ID (NCBI) 3586
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Protocols

Product Specific Protocols
WB protocol for IL-10 antibody 60269-1-IgDownload protocol
IHC protocol for IL-10 antibody 60269-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Front Immunol

Comparative Immunopathology of Cetacean morbillivirus Infection in Free-Ranging Dolphins From Western Mediterranean, Northeast-Central, and Southwestern Atlantic.

Authors - Josué Díaz-Delgado
ratWB

J Oral Rehabil

Molecular changes in peripheral blood involving osteoarthritic joint remodelling.

Authors - Hong-Yun Zhang
ratWB,IHC

BMJ Open Diabetes Res Care

KGF-2 and FGF-21 poloxamer 407 hydrogel coordinates inflammation and proliferation homeostasis to enhance wound repair of scalded skin in diabetic rats.

Authors - Xuanxin Yang
humanFC

DNA Cell Biol

Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis.

Authors - Yuan-Hao Wu
ratIF

Physiol Behav

Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria.

Authors - Carolina R Cawthon
ratWB

Mol Immunol

Bisperoxovanadium induces M2-type macrophages and promotes functional recovery after spinal cord injury.

Authors - Jia Liu