IL-17 Monoclonal antibody
IL-17 Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1B3D5
Cat no : 66148-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-937 cells, Jurkat cells, Raji cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
Product Information
The immunogen of 66148-1-Ig is IL-17 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | IL-17 fusion protein Ag3733 |
Full Name | interleukin 17A |
Calculated molecular weight | 155 aa, 18 kDa |
Observed molecular weight | 18 kDa |
GenBank accession number | BC067505 |
Gene symbol | IL17A |
Gene ID (NCBI) | 3605 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for IL-17 antibody 66148-1-Ig | Download protocol |
FC protocol for IL-17 antibody 66148-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
In Vitro Cell Dev Biol Anim MiR-let-7d-3p regulates IL-17 expression through targeting AKT1/mTOR signaling in CD4+ T cells. | ||
Exp Eye Res Glucocorticoid-induced leucine zipper overexpression inhibits lipopolysaccharide-induced retinal inflammation in rats. | ||
Biomed Pharmacother P-STAT3 and IL-17 in tumor tissues enhances the prognostic value of CEA and CA125 in patients with lung adenocarcinoma. |