IL-17D Monoclonal antibody
IL-17D Monoclonal Antibody for WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1D7H4
Cat no : 66143-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Recombinant protein, Recombinant protein protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
The immunogen of 66143-1-Ig is IL-17D Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IL-17D fusion protein Ag6762 |
| Full Name | interleukin 17D |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC036243 |
| Gene Symbol | IL17D |
| Gene ID (NCBI) | 53342 |
| RRID | AB_2881540 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL17D is a secreted cytokine with homology to the IL-17 family of proteins. IL17D is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas. The treatment of endothelial cells with IL17D has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by IL17D was found to be NF-kappa B-dependent.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-17D antibody 66143-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Ruyiping extract reduces lung metastasis in triple negative breast cancer by regulating macrophage polarization. | ||
Toxicology Nuclear factor erythroid 2-related factor 2 potentiates the generation of inflammatory cytokines by intestinal epithelial cells during hyperoxia by inducing the expression of interleukin 17D. | ||
Int Immunopharmacol ROS-activated MAPK/ERK pathway regulates crosstalk between Nrf2 and Hif-1α to promote IL-17D expression protecting the intestinal epithelial barrier under hyperoxia |
