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IL1 beta Antibody 76 Publications

Rabbit Polyclonal| Catalog number: 16806-1-AP

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Con: 29 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

IL1 beta Antibody WB Transfected HEK-293 cells 16806-1-AP

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 16806-1-AP( IL1 beta antibody at dilution of 1:700 incubated at room temperature for 1.5 hours

IL1 beta Antibody IHC human skin tissue 16806-1-AP

Immunohistochemistry of paraffin-embedded human skin tissue slide using 16806-1-AP( IL1 beta antibody at dilution of 1:200 (under 10x lens).

IL1 beta Antibody IHC human skin tissue 16806-1-AP

Immunohistochemistry of paraffin-embedded human skin tissue slide using 16806-1-AP( IL1 beta antibody at dilution of 1:200 (under 40x lens).

IL1 beta Antibody WB A431 cells 16806-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 16806-1-AP( IL1 beta antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

IL1 beta Antibody IF HeLa cells 16806-1-AP

Immunofluorescent analysis of HeLa cells using 16806-1-AP(IL1 beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human
Positive WB detected in:
A431 cells, Transfected HEK-293 cells
Positive IHC detected in:
human skin tissue, human tonsillitis tissue, human kidney tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IL1 beta fusion protein Ag10295
Full name:
interleukin 1, beta
Calculated molecular weight:
269aa,31 kDa
Observed molecular weight:
30-35 kDa
GenBank accession number:
BC008678
Gene ID (NCBI):
3553
Gene symbol
IL1B
Synonyms
IL1 beta, Catabolin, IL 1, IL 1 beta, IL1 BETA, IL1B, IL1beta, IL1F2, IL-1β, Interleukin 1 beta, interleukin 1, beta, Pro IL-1β

Background

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.


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