IL1 beta Antibody
IL1 beta Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
159
Cat no : 16806-1-AP
Synonyms
IL1 beta, Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL1beta, IL1F2, IL-1β, Interleukin 1 beta, interleukin 1, beta, Pro IL-1β
Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, A431 cells, human adrenal gland tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 130 publications below |
| IHC | See 25 publications below |
| IF | See 8 publications below |
Product Information
The 16806-1-AP immunogen is Fusion Protein IL1 beta protein expressed in E. coli.
| Reactivity | human |
| Cited Species | human, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IL1 beta fusion protein Ag10295 |
| Full Name | interleukin 1, beta |
| Calculated molecular weight | 269aa,31 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC008678 |
| Gene symbol | IL1B |
| Gene ID (NCBI) | 3553 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20oC. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL1 beta antibody 16806-1-AP | Download protocol |
| IHC protocol for IL1 beta antibody 16806-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress. | ||
J Cell Physiol Purple sweet potato color attenuated NLRP3 inflammasome by inducing autophagy to delay endothelial senescence. | ||
Front Pharmacol Protective Effects of Costunolide Against D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice. | ||
Int Immunopharmacol Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide/d-galactosamine induced acute liver failure in mice. | ||
Exp Ther Med Relationship between serum IL-1β and insomnia and liver depression in patients with perimenopausal non-organic sleep disorder. | ||
Antioxid Redox Signal GENDER DIFFERENCE IN DAMAGE MEDIATED SIGNALING CONTRIBUTES TO PULMONARY ARTERIAL HYPERTENSION. |
