|Positive WB detected in||LPS and Protein transport inhibitor treated THP-1 cells, human adrenal gland tissue, A431 cells|
|Positive IHC detected in||human tonsillitis tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 16806-1-AP is IL-1 Beta Fusion Protein expressed in E. coli.
|Cited Reactivity||canine, human, rabbit, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IL-1 Beta fusion protein Ag10295|
|Full Name||interleukin 1, beta|
|Calculated molecular weight||269 aa, 31 kDa|
|Observed molecular weight||30-35 kDa|
|GenBank accession number||BC008678|
|Gene ID (NCBI)||3553|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.
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