IL-1 Beta Polyclonal antibody
IL-1 Beta Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IF
Conjugate
Unconjugated
260
Cat no : 16806-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LPS and Protein transport inhibitor treated THP-1 cells, human adrenal gland tissue, A431 cells |
| Positive IHC detected in | human tonsillitis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 212 publications below |
| IHC | See 41 publications below |
| IF | See 11 publications below |
| ELISA | See 2 publications below |
Product Information
The immunogen of 16806-1-AP is IL-1 Beta Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | canine, human, rabbit, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IL-1 Beta fusion protein Ag10295 |
| Full Name | interleukin 1, beta |
| Calculated molecular weight | 269 aa, 31 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC008678 |
| Gene symbol | IL1B |
| Gene ID (NCBI) | 3553 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-1 Beta antibody 16806-1-AP | Download protocol |
| IHC protocol for IL-1 Beta antibody 16806-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress. | ||
J Cell Physiol Purple sweet potato color attenuated NLRP3 inflammasome by inducing autophagy to delay endothelial senescence. | ||
Front Pharmacol Protective Effects of Costunolide Against D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice. | ||
Int Immunopharmacol Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide/d-galactosamine induced acute liver failure in mice. | ||
Exp Ther Med Relationship between serum IL-1β and insomnia and liver depression in patients with perimenopausal non-organic sleep disorder. | ||
Antioxid Redox Signal GENDER DIFFERENCE IN DAMAGE MEDIATED SIGNALING CONTRIBUTES TO PULMONARY ARTERIAL HYPERTENSION. |
