ST2 Polyclonal antibody

ST2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11920-1-AP

Synonyms

DER4, FIT 1, IL1RL1, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1



Tested Applications

Positive WB detected inmouse brain tissue, multi-cells
Positive IHC detected inhuman lung cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11920-1-AP targets ST2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST2 fusion protein Ag2555
Full Name interleukin 1 receptor-like 1
Calculated molecular weight 63 kDa, 37 kDa, 30 kDa
Observed molecular weight 30 kDa, 40 kDa
GenBank accession numberBC030975
Gene symbol IL1RL1
Gene ID (NCBI) 9173
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein ST2, also known as IL1RL1, is a member of the interleukin 1 receptor family. The gene of ST2 encodes three splice variants: a soluble secreted form, a transmembrane form and a form of undetermined localization.This antibody recognizes at least 80kd, 40kd and 30kd forms in different tissues and cells.

Protocols

Product Specific Protocols
WB protocol for ST2 antibody 11920-1-APDownload protocol
IHC protocol for ST2 antibody 11920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

Interleukin-33 prevents the development of autoimmune diabetes in NOD mice.

Authors - Jingli Lu
ratIHC

J Mol Cell Cardiol

Yin-Yang 1 transcription factor modulates ST2 expression during adverse cardiac remodeling post-myocardial infarction.

Authors - M C Asensio-Lopez
humanIF

Cells

Helicobacter pylori Induces IL-33 Production and Recruits ST-2 to Lipid Rafts to Exacerbate Inflammation.

Authors - Chia-Jung Kuo
humanIHC

Circ Heart Fail

Pulmonary Production of Soluble ST2 in Heart Failure.

Authors - Domingo A Pascual-Figal
humanWB

Med Sci Monit

Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis.

Authors - Lijie Chen
humanIF

Clin Exp Dermatol

Interleukin-33 affects cytokine production by keratinocytes in vitiligo.

Authors - P Li