IL-2 Antibody

IL-2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11C5

Cat no : 60306-1-Ig

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF



Tested Applications

Positive WB detected inJurkat cells, Human white blood cells (WBC)
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

60306-1-Ig targets IL-2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-2 fusion protein Ag10199
Full Name interleukin 2
Calculated molecular weight 153aa,18 kDa
Observed molecular weight 18 kDa
GenBank accession number BC070338
Gene symbol IL2
Gene ID (NCBI) 3558
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

Protocols

Product Specific Protocols
WB protocol for IL-2 antibody 60306-1-IgDownload protocol
IHC protocol for IL-2 antibody 60306-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Free Radic Biol Med

Oxidative stress-induced IL-15 transtrans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo.

Authors - Xuguang Chen
mouseWB

PLoS One

N-Acetylcysteine Attenuates Ischemia-Reperfusion-Induced Apoptosis and Autophagy in Mouse Liver via Regulation of the ROS/JNK/Bcl-2 Pathway.

Authors - Chengfen Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alex (Verified Customer) (10-11-2019)

IL-2 Staining is dirty in mouse colon, attempted various dilutions and retrieval methods none of which worked.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse Colon
IL-2 Antibody Immunohistochemistry, validation (1:2000 dilution) in Mouse Colon (Cat no:60306-1-Ig)