Tested Applications
| Positive IHC detected in | human tonsillitis tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22859-1-AP targets IL-31 in IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18848 Product name: Recombinant human IL-31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-164 aa of BC132998 Sequence: RLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTLPCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTISQQFSECMDLALKSLTSGAQQATT Predict reactive species |
| Full Name | interleukin 31 |
| Calculated Molecular Weight | 164 aa, 18 kDa |
| GenBank Accession Number | BC132998 |
| Gene Symbol | IL-31 |
| Gene ID (NCBI) | 386653 |
| RRID | AB_2879179 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6EBC2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL-31 mRNA encodes a 164-aa precursor and a predicted 141-aa mature polypeptide, displaying an apparent molecular mass of 24 kDa in its glycosylated form. IL31 is a newly discovered four-helix bundle cytokine and expressed by several types of cells under both normal and disease state, such as activated CD4+T cells, mast cells, monocytes, macrophages, immature and mature monocyte-derived dendritic cells and so on. IL-31 signals through a heterodimeric receptor complex consisting of the IL31 receptor alpha (IL31Rα) and the oncostatin M receptor (OSMR). IL-31 plays a predominant function in the mediation of inflammatory and lymphoma-associated itch. Increased IL-31 expression has been detected in inflamed tissues from patients with atopic dermatitis, bowel diseases, allergic asthma and rhinitis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-31 antibody 22859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-IL-31 antibody (Cat# 22859-1-AP) has 4 total citations published in Scientific Reports, Neoplasia, World Journal of Gastrointestinal Oncology, and Journal of Cutaneous Pathology. The associated research fields include asthma, gastric cancer, and lichen planopilaris (dermatology), with applications spanning IHC, IF, and ELISA in human samples.
| Species | Application | Title |
|---|---|---|
Neoplasia OSMR coordinates a self-perpetuating circuit linking chemoresistance and neutrophil-driven immunosuppression in gastric cancer. | ||
World J Gastrointest Oncol Clinical implications of interleukins-31, 32, and 33 in gastric cancer | ||
J Cutan Pathol Evaluation of IL-31 and IL-31RA Expression in Lichen Planopilaris: A Retrospective Immunohistochemistry Study. |
