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IL33 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 12372-1-AP

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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IL33 Antibody IHC human tonsillitis tissue 12372-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12372-1-AP (IL33 antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IL33 Antibody IHC human tonsillitis tissue 12372-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12372-1-AP (IL33 antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive IHC detected in:
human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
IHC : 1:200-1:800
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IL33 fusion protein Ag2957
Full name:
interleukin 33
Calculated molecular weight:
30 kDa
Observed molecular weight:
GenBank accession number:
BC047085
Gene ID (NCBI):
90865
Gene symbol
IL33
Synonyms
C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

Background

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.


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