IL-5 Antibody

IL-5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26677-1-AP

Synonyms

EDF, IL 5, IL5, IL-5, Interleukin 5, T cell replacing factor, TRF



Tested Applications

Positive WB detected inRecombinant protein
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

The 26677-1-AP immunogen is Fusion Protein IL-5 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-5 fusion protein Ag24583
Full Name interleukin 5 (colony-stimulating factor, eosinophil)
Calculated molecular weight 134aa,15 kDa
Observed molecular weight
GenBank accession number BC066282
Gene symbol IL5
Gene ID (NCBI) 3567
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IL-5 antibody 26677-1-APDownload protocol
IHC protocol for IL-5 antibody 26677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Laryngoscope

Eosinophilic Sialodochitis: A Type of Chronic Obstructive Sialadenitis Related to Allergy.

Authors - Wen-Xuan Zhu