- Featured Product
- KD/KO Validated
IL-6R Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 23457-1-AP
CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL-6 R alpha, IL-6R, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80
|Positive WB detected in||Jurkat cells, mouse spleen tissue, rat spleen tissue|
|Positive IP detected in||mouse spleen tissue|
|Positive IHC detected in||human tonsillitis tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The 23457-1-AP immunogen is Fusion Protein IL-6R protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IL-6R fusion protein Ag18263|
|Full Name||interleukin 6 receptor|
|Calculated molecular weight||468aa,52 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC132684|
|Gene ID (NCBI)||3570|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R has been found in human serum and urine (PMID: 2529343; 1545125).
|Product Specific Protocols|
|WB protocol for IL-6R antibody 23457-1-AP||Download protocol|
|IHC protocol for IL-6R antibody 23457-1-AP||Download protocol|
|IF protocol for IL-6R antibody 23457-1-AP||Download protocol|
|IP protocol for IL-6R antibody 23457-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Kaohsiung J Med Sci
MiR-22 restrains proliferation of rheumatoid arthritis by targeting IL6R and may be concerned with the suppression of NF-κB pathway.
Death receptor 6 (DR6) is required for mouse B16 tumor angiogenesis via the NF-κB, P38 MAPK and STAT3 pathways.
J Biol Chem
Inducible microRNA-590-5p inhibits host antiviral response by targeting the soluble IL-6 receptor.