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  • KD/KO Validated

IL-6R Antibody

IL-6R Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23457-1-AP

Synonyms

CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL-6 R alpha, IL-6R, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80



Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 23457-1-AP immunogen is Fusion Protein IL-6R protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-6R fusion protein Ag18263
Full Name interleukin 6 receptor
Calculated molecular weight 468aa,52 kDa
Observed molecular weight 80 kDa
GenBank accession number BC132684
Gene symbol IL6R
Gene ID (NCBI) 3570
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R has been found in human serum and urine (PMID: 2529343; 1545125).

Protocols

Product Specific Protocols
WB protocol for IL-6R antibody 23457-1-APDownload protocol
IHC protocol for IL-6R antibody 23457-1-APDownload protocol
IF protocol for IL-6R antibody 23457-1-APDownload protocol
IP protocol for IL-6R antibody 23457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Kaohsiung J Med Sci

MiR-22 restrains proliferation of rheumatoid arthritis by targeting IL6R and may be concerned with the suppression of NF-κB pathway.

Authors - Qing-Yi Yang
mouseWB

Oncogenesis

Death receptor 6 (DR6) is required for mouse B16 tumor angiogenesis via the NF-κB, P38 MAPK and STAT3 pathways.

Authors - X Yang
humanWB

J Biol Chem

Inducible microRNA-590-5p inhibits host antiviral response by targeting the soluble IL-6 receptor.

Authors - Yaqin Zhou
  • KO Validated