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  • KD/KO Validated

INSIG1 Polyclonal antibody

INSIG1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22115-1-AP

Synonyms

CL 6, INSIG 1, INSIG1, insulin induced gene 1, Insulin induced gene 1 protein



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, SGC-7901 cells, MCF-7 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22115-1-AP is INSIG1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INSIG1 fusion protein Ag17420
Full Name insulin induced gene 1
Calculated Molecular Weight 277 aa, 30 kDa
Observed Molecular Weight 28-31 kDa, 66-70 kDa
GenBank Accession NumberBC001880
Gene Symbol INSIG1
Gene ID (NCBI) 3638
RRIDAB_11183051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).

Protocols

Product Specific Protocols
WB protocol for INSIG1 antibody 22115-1-APDownload protocol
IHC protocol for INSIG1 antibody 22115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nature

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Authors - Daqian Xu
  • KD Validated
WB

Transl Psychiatry

A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.

Authors - H L Cai
WB

Eur Neuropsychopharmacol

Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: Involvement of the INSIG/SREBP pathway.

Authors - Ruili Dang
WB

Mol Nutr Food Res

(-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet(†).

Authors - Hui Cheng
WB,IHC

J Physiol Biochem

Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention.

Authors - Shasha Liu
humanIF

JCI Insight

Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression.

Authors - Yingying Xu