IRAK4 Polyclonal antibody

IRAK4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18221-1-AP

Synonyms

IPD1, IRAK 4, IRAK4, NY REN 64, REN64



Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 18221-1-AP is IRAK4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRAK4 fusion protein Ag12982
Full Name interleukin-1 receptor-associated kinase 4
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC013316
Gene symbol IRAK4
Gene ID (NCBI) 51135
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IRAK4 antibody 18221-1-APDownload protocol
IHC protocol for IRAK4 antibody 18221-1-APDownload protocol
IP protocol for IRAK4 antibody 18221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Evid Based Complement Alternat Med

Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-κB Activation.

Authors - Xiangbing Shu
sheepWB

Mediators Inflamm

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Authors - Zhongjia Jiang