IRAK4 Polyclonal antibody

IRAK4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18221-1-AP

Synonyms

IPD1, IRAK 4, IRAK4, NY REN 64, REN64



Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

The immunogen of 18221-1-AP is IRAK4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRAK4 fusion protein Ag12982
Full Name interleukin-1 receptor-associated kinase 4
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC013316
Gene Symbol IRAK4
Gene ID (NCBI) 51135
RRIDAB_2878519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IRAK4 antibody 18221-1-APDownload protocol
IHC protocol for IRAK4 antibody 18221-1-APDownload protocol
IP protocol for IRAK4 antibody 18221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Mol Neurobiol

Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment

Authors - Liyun Deng
WB

Front Pharmacol

Euphorbia Factor L2 ameliorates the Progression of K/BxN Serum-Induced Arthritis by Blocking TLR7 Mediated IRAK4/IKKβ/IRF5 and NF-kB Signaling Pathways.

Authors - Jing Tang
sheepWB

Mediators Inflamm

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Authors - Zhongjia Jiang
WB

Evid Based Complement Alternat Med

Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-κB Activation.

Authors - Xiangbing Shu
humanWB

Int Immunopharmacol

Herbal compound cepharanthine attenuates inflammatory arthritis by blocking macrophage M1 polarization

Authors - Chenyang Lu