|Positive WB detected in||human blood, rat blood, mouse spleen tissue|
|Positive IP detected in||human plasma tissue|
|Positive IHC detected in||human spleen tissue, mouse spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24552-1-AP is CD41/Integrin Alpha 2B Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD41/Integrin Alpha 2B fusion protein Ag19459|
|Full Name||integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)|
|Calculated molecular weight||1039 aa, 113 kDa|
|Observed molecular weight||125 kDa|
|GenBank accession number||BC126442|
|Gene ID (NCBI)||3674|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light (25 kDa) and heavy (125 kDa) chains which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrin alpha-IIb/beta-3 is crucial for platelet aggregation through binding of soluble fibrinogen.
|Product Specific Protocols|
|WB protocol for CD41/Integrin Alpha 2B antibody 24552-1-AP||Download protocol|
|IHC protocol for CD41/Integrin Alpha 2B antibody 24552-1-AP||Download protocol|
|IP protocol for CD41/Integrin Alpha 2B antibody 24552-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cangrelor alleviates bleomycin-induced pulmonary fibrosis by inhibiting platelet activation in mice.
The role of necroptosis and apoptosis through the oxidative stress pathway in the liver of selenium-deficient swine.
Si-Miao-Yong-An Decoction Protects Against Cardiac Hypertrophy and Dysfunction by Inhibiting Platelet Aggregation and Activation.
J Transl Med
Platycodin D inhibits platelet function and thrombus formation through inducing internalization of platelet glycoprotein receptors.
RhTyrRS (Y341A), a novel human tyrosyl-tRNA synthetase mutant, stimulates thrombopoiesis through activation of the VEGF-R II/NF-κB pathway.
Disruption of Chromosomal Architecture of cox2 Locus Sensitizes Lung Cancer Cells to Radiotherapy.