CD41/Integrin Alpha 2B Polyclonal antibody

CD41/Integrin Alpha 2B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24552-1-AP

Synonyms

CD41, CD41B, GP2B, GPalpha IIb, GPIIb, GTA, HPA3, Integrin alpha IIb, ITGA2B, ITGAB



Tested Applications

Positive WB detected inhuman blood, rat blood, mouse spleen tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman spleen tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24552-1-AP is CD41/Integrin Alpha 2B Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivity human, mouse, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD41/Integrin Alpha 2B fusion protein Ag19459
Full Name integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
Calculated molecular weight 1039 aa, 113 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC126442
Gene symbol ITGA2B
Gene ID (NCBI) 3674
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light (25 kDa) and heavy (125 kDa) chains which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrin alpha-IIb/beta-3 is crucial for platelet aggregation through binding of soluble fibrinogen.

Protocols

Product Specific Protocols
WB protocol for CD41/Integrin Alpha 2B antibody 24552-1-APDownload protocol
IHC protocol for CD41/Integrin Alpha 2B antibody 24552-1-APDownload protocol
IP protocol for CD41/Integrin Alpha 2B antibody 24552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Mol Immunol

Cangrelor alleviates bleomycin-induced pulmonary fibrosis by inhibiting platelet activation in mice.

Authors - Tianwei Zhan
swineWB

Metallomics

The role of necroptosis and apoptosis through the oxidative stress pathway in the liver of selenium-deficient swine.

Authors - Yuan Zhang
mouseWB

Front Pharmacol

Si-Miao-Yong-An Decoction Protects Against Cardiac Hypertrophy and Dysfunction by Inhibiting Platelet Aggregation and Activation.

Authors - Congping Su
mouseWB

J Transl Med

Platycodin D inhibits platelet function and thrombus formation through inducing internalization of platelet glycoprotein receptors.

Authors - Qi Luo
humanWB

Biochem Pharmacol

RhTyrRS (Y341A), a novel human tyrosyl-tRNA synthetase mutant, stimulates thrombopoiesis through activation of the VEGF-R II/NF-κB pathway.

Authors - Yun Shi
humanWB

Mol Ther

Disruption of Chromosomal Architecture of cox2 Locus Sensitizes Lung Cancer Cells to Radiotherapy.

Authors - Yuxiang Sun