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  • KD/KO Validated

Integrin alpha-4 Antibody

Integrin alpha-4 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 19676-1-AP

Synonyms

CD49D, IA4, Integrin alpha 4, Integrin alpha IV, ITGA4, VLA 4 subunit alpha



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

19676-1-AP targets Integrin alpha-4 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Reactivity human
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
Calculated molecular weight 115 kDa
Observed molecular weight 140-150 kDa
GenBank accession number NM_000885
Gene symbol ITGA4
Gene ID (NCBI) 3676
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. I It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

Protocols

Product Specific Protocols
WB protocol for Integrin alpha-4 antibody 19676-1-APDownload protocol
IHC protocol for Integrin alpha-4 antibody 19676-1-APDownload protocol
FC protocol for Integrin alpha-4 antibody 19676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

Membrane microvesicles as mediators for melanoma-fibroblasts communication: roles of the VCAM-1/VLA-4 axis and the ERK1/2 signal pathway.

Authors - Xiao-Ping Zhao
  • KD Validated
mouseWB

J Nanobiotechnology

Macrophage-cancer hybrid membrane-coated nanoparticles for targeting lung metastasis in breast cancer therapy.

Authors - Chunai Gong
humanWB

Nat Commun

Calreticulin and integrin alpha dissociation induces anti-inflammatory programming in animal models of inflammatory bowel disease.

Authors - Masayoshi Ohkuro

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (03-11-2019)

For WB, the expected band was very weak, and was much weaker than those non-specific bands; for IF, it could label the cell cortex localisation of Itga4, partially co-localised with Actin.

  • Applications: Western Blot,Immunofluorescence,
  • Cell Tissue Type: MEF
No image submitted with review