Integrin alpha-V Polyclonal antibody

Integrin alpha-V Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27096-1-AP

Synonyms

CD51, Integrin alpha V, ITGAV, MSK8, VNRA



Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, HUVEC cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27096-1-AP is Integrin alpha-V Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin alpha-V fusion protein Ag25825
Full Name integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
Calculated molecular weight 1048 aa, 116 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC136442
Gene symbol ITGAV
Gene ID (NCBI) 3685
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Integrin alpha-V antibody 27096-1-APDownload protocol
IHC protocol for Integrin alpha-V antibody 27096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Cell Int

Bioinformatics combined with quantitative proteomics analyses and identification of potential biomarkers in cholangiocarcinoma.

Authors - Zijian Da
humanWB

Int J Mol Sci

Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients.

Authors - Emma Plana

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody gave a clean band at the expected molecular weight; by IF, it could not label a specific pattern.

  • Applications: Western Blot,Immunofluorescence,
  • Cell Tissue Type: MEF