|Positive WB detected in||MCF-7 cells, A549 cells, HUVEC cells|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
The immunogen of 27096-1-AP is Integrin alpha-V Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Integrin alpha-V fusion protein Ag25825|
|Full Name||integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)|
|Calculated molecular weight||1048 aa, 116 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC136442|
|Gene ID (NCBI)||3685|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (04-24-2019)
By WB, this antibody gave a clean band at the expected molecular weight; by IF, it could not label a specific pattern.