CD11c/Integrin Alpha X Polyclonal antibody

CD11c/Integrin Alpha X Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17342-1-AP

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6



Tested Applications

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17342-1-AP targets CD11c/Integrin Alpha X in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD11c/Integrin Alpha X fusion protein Ag11380
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight 1169 aa, 129 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC038237
Gene symbol ITGAX
Gene ID (NCBI) 3687
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Protocols

Product Specific Protocols
WB protocol for CD11c/Integrin Alpha X antibody 17342-1-APDownload protocol
IHC protocol for CD11c/Integrin Alpha X antibody 17342-1-APDownload protocol
FC protocol for CD11c/Integrin Alpha X antibody 17342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Protective Effects of Live Combined B. subtilis and E. faecium in Polymicrobial Sepsis Through Modulating Activation and Transformation of Macrophages and Mast Cells.

Authors - Lisha Guo
mouseWB,IHC

J Cell Mol Med

PTPN2 improved renal injury and fibrosis by suppressing STAT-induced inflammation in early diabetic nephropathy.

Authors - Ya Li
mouseIHC

J Diabetes Res

Attenuation of Diabetic Nephropathy in Diabetic Mice by Fasudil through Regulation of Macrophage Polarization.

Authors - Fajiang Xie
humanIHC

Int Immunopharmacol

(+)-Borneol improves the efficacy of edaravone against DSS-induced colitis by promoting M2 macrophages polarization via JAK2-STAT3 signaling pathway.

Authors - Xiong Zhang
humanIHC

Eur J Gastroenterol Hepatol

Indoleamine 2,3-dioxygenase expression in human inflammatory bowel disease.

Authors - Zhou Liping L
humanIF

Int J Legal Med

Forensic pathological study on temporal appearance of dendritic cells in skin wounds.

Authors - Yumi Kuninaka