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CD11c/Integrin alpha X Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 17342-1-AP

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Tested Applications
Applications:
ELISA, WB, IHC, FC
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17342-1-AP;human plasma tissue

human blood tissue were subjected to SDS PAGE followed by western blot with 17342-1-AP(ITGAX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

17342-1-AP;human tonsillitis tissue

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP( CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

17342-1-AP;human tonsillitis tissue

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP( CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

17342-1-AP;HL-60 cells

1X10^6 HL-60 cells were stained with 0.2ug CD11c/Integrin alpha X antibody (17342-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
human blood tissue
Positive IHC detected in:
human tonsillitis tissue, human spleen tissue, human tonsillitis tissue
Positive FC detected in:
HL-60 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD11c/Integrin alpha X fusion protein ag11380
Full name:
integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight:
1169aa,129 kDa
Observed molecular weight:
150 kDa
GenBank accession number:
BC038237
Gene ID (NCBI):
3687
Gene symbol
ITGAX
Synonyms
CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6
Background

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a type I transmembrane protein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.


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