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CD11c/Integrin alpha X Antibody 11 Publications

Rabbit Polyclonal| Catalog number: 17342-1-AP

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

CD11c/Integrin alpha X Antibody WB human plasma tissue 17342-1-AP

human blood were subjected to SDS PAGE followed by western blot with 17342-1-AP(ITGAX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

CD11c/Integrin alpha X Antibody IHC human tonsillitis tissue 17342-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP( CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD11c/Integrin alpha X Antibody IHC human tonsillitis tissue 17342-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP( CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD11c/Integrin alpha X Antibody FC HL-60 cells 17342-1-AP

1X10^6 HL-60 cells were stained with 0.2ug CD11c/Integrin alpha X antibody (17342-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse
Positive WB detected in:
human blood tissue
Positive IHC detected in:
human tonsillitis tissue, mouse spleen tissue, human spleen tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
HL-60 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD11c/Integrin alpha X fusion protein Ag11380
Full name:
integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight:
1169aa,129 kDa
Observed molecular weight:
150 kDa
GenBank accession number:
BC038237
Gene ID (NCBI):
3687
Gene symbol
ITGAX
Synonyms
CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

Background

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.


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