CD61 / Integrin Beta 3 Polyclonal antibody

CD61 / Integrin Beta 3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18309-1-AP

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, Integrin beta-3, ITGB3



Tested Applications

Positive WB detected inHuman peripheral blood platelets, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18309-1-AP targets CD61 / Integrin Beta 3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC127667
Gene Symbol ITGB3
Gene ID (NCBI) 3690
RRIDAB_2128759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

Protocols

Product Specific Protocols
WB protocol for CD61 / Integrin Beta 3 antibody 18309-1-APDownload protocol
IP protocol for CD61 / Integrin Beta 3 antibody 18309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Platelet-derived porous nanomotor for thrombus therapy.

Authors - Mimi Wan
WB

Nat Commun

Targeting Gα13-integrin interaction ameliorates systemic inflammation.

Authors - Ni Cheng
human,mouseIF

Biomaterials

Construction and evaluation of novel αvβ3 integrin ligand-conjugated ultrasmall star polymer micelles targeted glomerular podocytes through GFB permeation.

Authors - Chengyuan Huang
ratWB

Theranostics

A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance.

Authors - Peipei Jin

Thromb Res

Repurposing of rilpivirine for preventing platelet β3 integrin-dependent thrombosis by targeting c-Src active autophosphorylation

Authors - Kui Liu
mouseIF,CoIP

NPJ Regen Med

hUC-MSCs-derived MFGE8 ameliorates locomotor dysfunction via inhibition of ITGB3/ NF-κB signaling in an NMO mouse model

Authors - Huiming Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sathyanarayani (Verified Customer) (04-05-2023)

I would highly recommend this primary antibody if you are looking for ITGB3. It produced really clear strong bands at 100kDa MW when used for western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse lysed blood
FH

Sathyanarayani (Verified Customer) (04-05-2023)

The ITGB3 antibody produced really nice clear bands on the western blot at 110Kda MW. I would highly recommend using this if you are measuring ITGB3.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse blood