Integrin Beta 3 Polyclonal antibody

Integrin Beta 3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18309-1-AP

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, Integrin beta-3, ITGB3



Tested Applications

Positive WB detected inHuman peripheral blood platelets, rat spleen tissue, mouse spleen tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

18309-1-AP targets Integrin Beta 3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Calculated molecular weight 87 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC127667
Gene symbol ITGB3
Gene ID (NCBI) 3690
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 3 antibody 18309-1-APDownload protocol
IP protocol for Integrin Beta 3 antibody 18309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Blood Adv

Intratumoral platelet aggregate formation in a murine preclinical glioma model depends on podoplanin expression on tumor cells.

Authors - Barbara Costa
humanWB

Nanotechnology

High transfection efficiency of quantum dot-antisense oligonucleotide nanoparticles in cancer cells through dual-receptor synergistic targeting.

Authors - Ming-Zhen Zhang
ratWB

J Tissue Eng Regen Med

PMID: 29498229

Authors - Rongyao Xu
humanWB

J Biol Chem

Integrin αvβ3-associated DAAM1 is essential for collagen-induced invadopodia extension and cell haptotaxis in breast cancer cells.

Authors - Ting Yan
human,ratWB

Invest Ophthalmol Vis Sci

PMID: 30128492

Authors - Na Wang
humanWB

Gynecol Endocrinol

Increased expression of Talin1 in the eutopic and ectopic endometria of women with adenomyosis.

Authors - Jianfa Jiang