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Integrin beta-4 Antibody

Integrin beta-4 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 21738-1-AP

Synonyms

CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4



Tested Applications

Positive WB detected inBxPC-3 cells, MCF-7 cells, A431 cells, A549 cells, HT-29 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

The 21738-1-AP immunogen is Fusion Protein Integrin beta-4 protein expressed in E. coli.

Reactivity human, mouse, rat
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin beta-4 fusion protein Ag16450
Full Name integrin, beta 4
Calculated molecular weight 1752aa,195 kDa
Observed molecular weight 200-240 kDa
GenBank accession number BC126411
Gene symbol ITGB4
Gene ID (NCBI) 3691
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Protocols

Product Specific Protocols
WB protocol for Integrin beta-4 antibody 21738-1-APDownload protocol
IHC protocol for Integrin beta-4 antibody 21738-1-APDownload protocol
IP protocol for Integrin beta-4 antibody 21738-1-APDownload protocol
FC protocol for Integrin beta-4 antibody 21738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Exp Clin Cancer Res

Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.

Authors - Binbin Ma
  • KD Validated
humanWB

J Cell Biochem

17-allylamino-17-demethoxygeldanamycin impeded chemotherapy through antioxidant activation via reducing reactive oxygen species-induced cell death.

Authors - Yueqi Li
humanWB,IHC,IF

Sci Rep

Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma.

Authors - Xiao-Long Li