Integrin Beta 1 Polyclonal antibody

Name: Integrin Beta 1 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 26918-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26918-1-AP

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

WB analysis of HT-1080 using 26918-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human skin using 26918-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skin using 26918-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens).

Cat No. 26918-1-AP

Freight/Packing: -

Country/Region

Size/Concentration

Quantity

Positive WB detected in	HT-1080 cells
Positive IHC detected in	human skin tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

WB	See 2 publications below
IF	See 2 publications below

The immunogen of 26918-1-AP is Integrin Beta 1 Fusion Protein expressed in E. coli.

Tested Reactivity	human
Cited Reactivity	Hamster, human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Integrin Beta 1 fusion protein Ag25426
Full Name	integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated molecular weight	88 kDa
Observed molecular weight	130 kDa
GenBank accession number	BC020057
Gene symbol	ITGB1
Gene ID (NCBI)	3688
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Product Specific Protocols
WB protocol for Integrin Beta 1 antibody 26918-1-AP	Download protocol
IHC protocol for Integrin Beta 1 antibody 26918-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
	WB	Theriogenology Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway. Authors - Xue Wang View Article
human	IF	Cancer Cell Int Silencing Formin-like 2 inhibits growth and metastasis of gastric cancer cells through suppressing internalization of integrins. Authors - Banghua Zhong View Article
Hamster	IF	J Virol Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus. Authors - Xiu Xin View Article
human	WB	Cancer Lett VCAM-1 secreted from cancer-associated fibroblasts enhances the growth and invasion of lung cancer cells through AKT and MAPK signaling. Authors - Zhuan Zhou View Article