Integrin beta-1 Antibody

Integrin beta-1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 26918-1-AP

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB



Tested Applications

Positive WB detected inHT-1080 cells
Positive IHC detected inhuman skin tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 26918-1-AP immunogen is Fusion Protein Integrin beta-1 protein expressed in E. coli.

Reactivity human
Cited Species Hamster, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin beta-1 fusion protein Ag25426
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated molecular weight 88 kDa
Observed molecular weight 130 kDa
GenBank accession number BC020057
Gene symbol ITGB1
Gene ID (NCBI) 3688
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Integrin beta-1 antibody 26918-1-APDownload protocol
IHC protocol for Integrin beta-1 antibody 26918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Theriogenology

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Authors - Xue Wang
humanIF

Cancer Cell Int

Silencing Formin-like 2 inhibits growth and metastasis of gastric cancer cells through suppressing internalization of integrins.

Authors - Banghua Zhong
HamsterIF

J Virol

Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.

Authors - Xiu Xin
humanWB

Cancer Lett

VCAM-1 secreted from cancer-associated fibroblasts enhances the growth and invasion of lung cancer cells through AKT and MAPK signaling.

Authors - Zhuan Zhou