Tested Applications
| Positive WB detected in | HT-1080 cells, A549 cells, HeLa cells, A431 cells, U-87 MG cells, HCT 116 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82729-3-RR targets Integrin Beta 1/CD29 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3078 Product name: Recombinant human Integrin beta-1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-359 aa of BC020057 Sequence: ANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGT Predict reactive species |
| Full Name | integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 110-140 kDa |
| GenBank Accession Number | BC020057 |
| Gene Symbol | Integrin beta 1 |
| Gene ID (NCBI) | 3688 |
| ENSEMBL Gene ID | ENSG00000150093 |
| RRID | AB_3670539 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05556 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Integrin Beta 1/CD29 antibody 82729-3-RR | Download protocol |
| IHC protocol for Integrin Beta 1/CD29 antibody 82729-3-RR | Download protocol |
| WB protocol for Integrin Beta 1/CD29 antibody 82729-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Integrin Beta 1/CD29 Recombinant monoclonal antibody (1K20), catalog number 82729-3-RR, has 1 citation to date. It was cited in Nature Communications (IF 18.1) in a 2025 study on self-assembly of hybrid 3D cultures integrating living and synthetic cells, falling within the fields of tissue engineering and synthetic biology.
| Species | Application | Title |
|---|---|---|
Nat Commun Self-assembly of hybrid 3D cultures by integrating living and synthetic cells. |
