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JAK2 Antibody

JAK2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17670-1-AP

Synonyms

JAK 2, JAK2, Janus kinase 2, JTK10, Tyrosine protein kinase JAK2



Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 17670-1-AP immunogen is Fusion Protein JAK2 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JAK2 fusion protein Ag11264
Full Name Janus kinase 2 (a protein tyrosine kinase)
Calculated molecular weight 1132aa,130 kDa
Observed molecular weight 150 kDa
GenBank accession number BC039695
Gene symbol JAK2
Gene ID (NCBI) 3717
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer. This antibody can detect full-length band of 130 kDa, and another bands 150 kDa after posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for JAK2 antibody 17670-1-APDownload protocol
IHC protocol for JAK2 antibody 17670-1-APDownload protocol
IP protocol for JAK2 antibody 17670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Brain Res Bull

Inhibition of Connexin43 Hemichannels with Gap19 Protect-s Cerebral Ischemia/Reperfusion Injury via the JAK2/STAT3 Pathway in Mice.

Authors - Beilei Chen
humanWB

J Immunol

FGFR2 Promotes Expression of PD-L1 in Colorectal Cancer via the JAK/STAT3 Signaling Pathway.

Authors - Piao Li
humanWB

Colloids Surf B Biointerfaces

Toxicological exploration of peptide-based cationic liposomes in siRNA delivery.

Authors - Yanyan Zhu
ratWB

Naunyn Schmiedebergs Arch Pharmacol

Protocatechuic aldehyde protects against isoproterenol-induced cardiac hypertrophy via inhibition of the JAK2/STAT3 signaling pathway.

Authors - Xiuli Fang

Am J Physiol Renal Physiol

Triptolide delays disease progression in an adult rat model of polycystic kidney disease through the JAK2/STAT3 pathway.

Authors - Ying Jing
mouseWB

Oxid Med Cell Longev

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Authors - Wei Wang