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JAK2 Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 17670-1-AP

Featured Product KD/KO validated

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Con: 51 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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JAK2 Antibody IHC human kidney tissue 17670-1-AP

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

JAK2 Antibody IHC human kidney tissue 17670-1-AP

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 17670-1-AP (JAK2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

JAK2 Antibody WB Jurkat cells 17670-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 17670-1-AP( JAK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

JAK2 Antibody IP Jurkat cells 17670-1-AP

IP Result of anti-JAK2 (IP:17670-1-AP, 4ug; Detection:17670-1-AP 1:300) with Jurkat cells lysate 3800ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, NIH/3T3 cells
Positive IP detected in:
Jurkat cells
Positive IHC detected in:
human kidney tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
JAK2 fusion protein Ag11264
Full name:
Janus kinase 2 (a protein tyrosine kinase)
Calculated molecular weight:
1132aa,130 kDa
Observed molecular weight:
150 kDa
GenBank accession number:
BC039695
Gene ID (NCBI):
3717
Gene symbol
JAK2
Synonyms
JAK 2, JAK2, Janus kinase 2, JTK10, Tyrosine protein kinase JAK2

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