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- KD/KO Validated
JAK2 Antibody
JAK2 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17670-1-AP
Synonyms
JAK 2, JAK2, Janus kinase 2, JTK10, Tyrosine protein kinase JAK2
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 28 publications below |
Product Information
The 17670-1-AP immunogen is Fusion Protein JAK2 protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | JAK2 fusion protein Ag11264 |
| Full Name | Janus kinase 2 (a protein tyrosine kinase) |
| Calculated molecular weight | 1132aa,130 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC039695 |
| Gene symbol | JAK2 |
| Gene ID (NCBI) | 3717 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer. This antibody can detect full-length band of 130 kDa, and another bands 150 kDa after posttranslational modification.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for JAK2 antibody 17670-1-AP | Download protocol |
| IHC protocol for JAK2 antibody 17670-1-AP | Download protocol |
| IP protocol for JAK2 antibody 17670-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Res Bull Inhibition of Connexin43 Hemichannels with Gap19 Protect-s Cerebral Ischemia/Reperfusion Injury via the JAK2/STAT3 Pathway in Mice. | ||
J Immunol FGFR2 Promotes Expression of PD-L1 in Colorectal Cancer via the JAK/STAT3 Signaling Pathway. | ||
Colloids Surf B Biointerfaces Toxicological exploration of peptide-based cationic liposomes in siRNA delivery. | ||
Naunyn Schmiedebergs Arch Pharmacol Protocatechuic aldehyde protects against isoproterenol-induced cardiac hypertrophy via inhibition of the JAK2/STAT3 signaling pathway. | ||
Am J Physiol Renal Physiol Triptolide delays disease progression in an adult rat model of polycystic kidney disease through the JAK2/STAT3 pathway. | ||
Oxid Med Cell Longev Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation. |
