JNK Polyclonal antibody

JNK Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10023-1-AP

Synonyms

c Jun N terminal kinase 1, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1



Tested Applications

Positive WB detected inNIH/3T3 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, rat spleen tissue, RAW 264.7 cells, RAW264.7, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human lung cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10023-1-AP targets JNK in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JNK fusion protein Ag0019
Full Name mitogen-activated protein kinase 8
Calculated molecular weight 48 kDa
Observed molecular weight 40-45 kDa, 50-55 kDa
GenBank accession numberNM_138982
Gene symbol MAPK8
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 – MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease
  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Protocols

Product Specific Protocols
WB protocol for JNK antibody 10023-1-APDownload protocol
IHC protocol for JNK antibody 10023-1-APDownload protocol
IF protocol for JNK antibody 10023-1-APDownload protocol
IP protocol for JNK antibody 10023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Pharmacol Res

Pharmacological preconditioning with the cellular stress inducer thapsigargin protects against experimental sepsis.

Authors - Yaping Wei
humanWB

Chem Res Toxicol

Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response and calcium release.

Authors - Demei Xu
ratWB

Cells

CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1.

Authors - Ji-Eun Kim
human

Front Oncol

Upregulation of OTUD7B (Cezanne) Promotes Tumor Progression via AKT/VEGF Pathway in Lung Squamous Carcinoma and Adenocarcinoma.

Authors - Dan-Dan Lin
humanWB

Chin Med J (Engl)

Focal adhesion kinase antisense oligodeoxynucleotides inhibit human pulmonary artery smooth muscle cells proliferation and promote human pulmonary artery smooth muscle cells apoptosis.

Authors - Lin Chun-long CL
WB

Theriogenology

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Authors - Xue Wang