JNK Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 10023-1-AP

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Freight/Packing

Con: 35 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
NIH/3T3 cells,Jurkat cells, HeLa cells, HEK-293 cells, RAW 264.7 cells, RAW264.7 , MCF-7 cells, SH-SY5Y cells, A431 cells, rat spleen tissue

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human prostate cancer tissue, human lung cancer tissue, mouse heart tissue

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
mitogen-activated protein kinase 8

Calculated molecular weight:
48 kDa

Observed molecular weight:
40-45 kDa, 50-55 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
MAPK8

Synonyms
c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1
Background

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ).


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