KARS Polyclonal antibody

KARS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14951-1-AP

Synonyms

KARS, KARS2, KIAA0070, KRS, Lysine tRNA ligase, LysRS, lysyl tRNA synthetase



Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, human colon tissue, HeLa cells, HepG2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14951-1-AP targets KARS in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KARS fusion protein Ag6778
Full Name lysyl-tRNA synthetase
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC004132
Gene symbol KARS
Gene ID (NCBI) 3735
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KARS antibody 14951-1-APDownload protocol
IHC protocol for KARS antibody 14951-1-APDownload protocol
IF protocol for KARS antibody 14951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Nucleic Acids Res

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Authors - Danni Chen
human,mouseWB

Sci Adv

Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation.

Authors - J Guerra
humanWB

Cell Chem Biol

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Authors - Li Wang
humanWB

Clin Genet

Novel mutations in KARS cause hypertrophic cardiomyopathy and combined mitochondrial respiratory chain defect.

Authors - Daniela Verrigni
humanIF

Front Cell Infect Microbiol

Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.

Authors - Tian Luo
humanWB

Hum Mutat

Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.

Authors - Benedetta Ruzzenente

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kishor (Verified Customer) (01-30-2019)

This antibody working well against rat liver tissue and human cancer cells

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue
KARS Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:14951-1-AP)